07-1285

Anti-acetylated-Src (Lys430) Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: SRC
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• clon: polyclonal
• application: WB; cell based assay
• reactividad de especies: human
• técnicas: cell based assay: suitable; western blot: suitable

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human
• reactividad de especies (predicha por homología): mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
• técnicas: cell based assay: suitable; western blot: suitable
• Nº de acceso NCBI: NP_005408
• Nº de acceso UniProt: P12931
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: acetylation (Lys430)
• Información sobre el gen: human ... SRC(6714)

Descripción

Descripción general

Src is a proto-oncogenic tyrosine kinase belonging to the Src family of non-receptor tyrosine kinases. This proto-oncogene plays a pivotal role cellular processes such as proliferation, migration, transformation and regulation of embyronic development and cell growth. Src tyrosine kinases transmit integrin-dependent signals for cell movement and proliferation. The study of Src proteins have contributed to the understanding of cancer caused by abnormal cellular signaling.

Especificidad

This antibody recognizes Src acetylated at Lys430.

Inmunógeno

Epitope: Acetylated Lys430

KLH-conjugated linear peptide corresponding to human Src acetylated at Lys430.

Aplicación

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

This Anti-acetylated-Src (Lys430) Antibody is validated for use in WB, Cell Function Assay for the detection of acetylated-Src (Lys430).

Western Blot Analysis: 5 µg/mL from a representative lot detected Src on 10 µg of HEK293T cells transfected with myc-tagged Src and CBP.

Calidad

Western Blot/Peptide Inhibition Assay Analysis: This antibody peptide blocked on HEK293T cells transfected with myc-tagged Src and CBP.

Descripción de destino

~69 kDa observed.
Endogenous Src has a molecular weight of ~60 kDa; however, the myc tag adds ~9 kDa to the weight of the protein.

Forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
HEK293T cells transfected with myc-tagged Src and CBP

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids