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Key Documents

07-1218

Sigma-Aldrich

Anti-PP1γ1 Antibody

Upstate®, from rabbit

Sinónimos:

Protein phosphatase 1C catalytic subunit, Serine/threonine-protein phosphatase PP1-gamma catalytic subunit, Protein phosphatase 1, catalytic subunit, gamma isoform, Serine/threonine phosphatase 1 gamma

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

forma del anticuerpo

affinity purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

polyclonal

reactividad de especies

bovine, mouse, rat, human

fabricante / nombre comercial

Upstate®

técnicas

immunoprecipitation (IP): suitable
western blot: suitable

isotipo

IgG

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... PPP1CC(5501)

Descripción general

Type 1 protein Ser/Thr phosphatases (PP1) have a high specific activity against phosphorylase, and are resistant to okadaic acid up to concentrations of 50 nM, but sensitive to Inhibitor-2 and microcystin LR.

Especificidad

Recognizes Protein Phosphatase 1γ1. No cross reactivity with other recombinant PP1 isoforms.

Inmunógeno

Epitope: C-terminus
The immunogen used to the purified peptide conjugated to KLH corresponding to the to the C –terminus of PP1 gamma 1 catalytic subunit (amino acids 311-323).

Aplicación

Anti-PP1γ1 Antibody detects level of PP1γ1 & has been published & validated for use in WB & IP.
Immunoprecipitation: A previous lot of this antibody was used on Immunoprecipitation.
Research Category
Signaling
Research Sub Category
Kinases & Phosphatases

Calidad

Routinely evaluated by Western Blot on HeLa lysates.

Western Blot Analysis: 1:500 dilution of this lot detected PP-1 GAMMA on 10 μg of HeLa lysates.

Descripción de destino

~37 kDa

Ligadura / enlace

Replaces: AB4084

Forma física

Format: Purified
Purified
Purified rabbit polyclonal IgG provided as 0.2µm sterile filtered solution in phosphate buffered saline with 0.08% sodium azide.

Almacenamiento y estabilidad

Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análisis

Control
HeLa Cell Lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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