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05-949

Sigma-Aldrich

Anti-Histidine Tagged Antibody, clone HIS.H8

clone HIS.H8, Upstate®, from mouse

Sinónimos:

HIS Tag Antibody

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.43

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

purified antibody

clon

HIS.H8, monoclonal

reactividad de especies (predicha por homología)

all

fabricante / nombre comercial

Upstate®

técnicas

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotipo

IgG2b

Condiciones de envío

dry ice

modificación del objetivo postraduccional

unmodified

Descripción general

His-Tag is a series of at least 6 histidine residues used as a tag motif at the N- or C-terminus of recombinant proteins. The relatively small size of this tag vs. other commonly used tags such as GST (30 kDa) makes it less likely to interfere with target protein structure or function. Its small size also makes it more suitable for use under denaturing conditions. His tags are most often used for affinity purification and binding assays. His tags bind to nickel or cobalt ions with high affinity, and this interaction with metal ions is the basis of many his-tag based affinity media. The His Tag epitope is widely used to detect expression of recombinant proteins in bacterial, yeast, insect,and mammalian systems.

Especificidad

Recognizes tag sequence HHHHHH on either the N- or C-terminus of any recombinant fusion protein; no other amino acids surrounding the 6x-His tag sequence are required for the recognition of the 6x-His tag.

Inmunógeno

6x His synthetic peptide.

Aplicación

Immunocytochemistry:
2 μg/mL of a previous lot of antibody showed positive immunostaining for His tagged Akt1/PKBα in transfected HeLa cells.

Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate His Tagged proteins.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

RNA Binding Protein (RBP)
This Anti-Histidine Tagged Antibody, clone HIS.H8 is validated for use in IC, IP, WB for the detection of Histidine Tagged.

Calidad

Routinely evaluated by western blot and immunocytochemistry on transfected HeLa cells.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected His tagged Akt1/PKBα in RIPA lysates of transfected HeLa cells.

Forma física

Format: Purified
Purified
Purified mouse monoclonal IgG2b in buffer containing PBS, pH 7.4 with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análisis

Control
RIPA lysates of transfected HeLa cells.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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