05-844

Anti-PLK1 Antibody, clone 35-206

clone 35-206, Upstate^®, from mouse

• Sinónimos: Polo-like kinase 1, Serine/threonine-protein kinase 13, cell cycle regulated protein kinase, polo (Drosophia)-like kinase, polo like kinase, polo like kinase, polo-like kinase (Drosophila), polo-like kinase 1 (Drosophila)
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100; 400
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 35-206, monoclonal
• reactividad de especies: rat, mouse, human
• fabricante / nombre comercial: Upstate^®
• técnicas: immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG2b
• Nº de acceso NCBI: NP_005021
• Nº de acceso UniProt: P53350
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... PLK1(5347) ; mouse ... Plk1(18817)

Descripción

Descripción general

Polo-like Kinase 1 (PLK-1) is a serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Expressed PLK-1 protein accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.

Especificidad

PLK1

Inmunógeno

6His-tagged full length fusion protein corresponding to human polo-like kinase 1 (PLK1). Also called serine-threonine protein kinase PLK.

Aplicación

Anti-PLK1 Antibody, clone 35-206 detects level of PLK1 & has been published & validated for use in WB, IP, IC, IH.

Immunoprecipitation: An independent laboratory has reported that a previous lot of this antibody can be used to immunoprecipitate PLK1.

Immunohisto/immunocytochemistry: An independent laboratory reported that 1 μg/mL of a previous lot of this antibody showed positive immunostaining for PLK1 in paraformaldehyde treated, Triton X-100 fixed cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Calidad

Evaluated by western blot on RIPA lysates from 3T3/A31 cells.

Western Blot Analysis: 0.5-2 μg/mL of this antibody detected PLK1 in RIPA lysates from 3T3/A31 cells.

Descripción de destino

64 kDa

Forma física

Format: Purified

Protein A purified

Protein A purified mouse IgG2b in buffer containing 0.07M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide and 30% glycerol. Store at -20°C.

Almacenamiento y estabilidad

Stable for 1 year at from date of receipt.

Nota de análisis

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1