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Merck
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Key Documents

05-808

Sigma-Aldrich

Anti-dimethyl-Histone H3 (Arg2) Antibody, rabbit monoclonal

Upstate®, from rabbit

Sinónimos:

H3R2me2, Histone H3 (di methyl R2)

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

monoclonal

reactividad de especies

human

fabricante / nombre comercial

Upstate®

técnicas

dot blot: suitable
inhibition assay: suitable (peptide)
multiplexing: suitable
western blot: suitable

isotipo

IgG

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

dimethylation (Arg2)

Información sobre el gen

human ... H3C1(8350)

Descripción general

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.



The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Inmunógeno

peptide containing the sequence AdimRTKQ, corresponding to dimethyl-arginine 2 of human histone H3.

Aplicación

Use Anti-dimethyl-Histone H3 (Arg2) Antibody (Rabbit Monoclonal Antibody) validated in DB, WB, PIA, Mplex to detect dimethyl-Histone H3 (Arg2) also known as H3R2me2, Histone H3 (di methyl R2).

Calidad

routinely evaluated in acid-extracted proteins from HeLa cells

Descripción de destino

17kDa

Forma física

Format: Purified

Nota de análisis

Control
Acid extracts from HeLa cells

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1


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Zichuan Liu et al.
Developmental biology, 368(2), 304-311 (2012-06-05)
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Lessly P Sepulveda-Rincon et al.
Biology of reproduction, 95(6), 123-123 (2016-10-21)
The first lineage specification during mammalian embryo development can be visually distinguished at the blastocyst stage. Two cell lineages are observed on the embryonic-abembryonic axis of the blastocyst: the inner cell mass and the trophectoderm. The timing and mechanisms driving
Alessandra Di Lorenzo et al.
Nucleic acids research, 42(13), 8297-8309 (2014-06-19)
Protein arginine methyltransferase 6 (PRMT6) is a nuclear enzyme that modifies histone tails. To help elucidate the biological function of PRMT6 in vivo, we generated transgenic mice that ubiquitously express PRMT6 fused to the hormone-binding portion of the estrogen receptor
Ayaz Ahmad et al.
PloS one, 6(8), e22664-e22664 (2011-08-20)
Post-translational methylation of arginine residues profoundly affects the structure and functions of protein and, hence, implicated in a myriad of essential cellular processes such as signal transduction, mRNA splicing and transcriptional regulation. Protein arginine methyltransferases (PRMTs), the enzymes catalyzing arginine
Analysis of histones in Xenopus laevis. I. A distinct index of enriched variants and modifications exists in each cell type and is remodeled during developmental transitions.
Shechter, D; Nicklay, JJ; Chitta, RK; Shabanowitz, J; Hunt, DF; Allis, CD
The Journal of Biological Chemistry null

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