05-807
Anti-phospho-CREB (Ser133) Antibody, clone 634-2
clone 634-2, Upstate®, from mouse
Sinónimos:
active transcription factor CREB, cAMP responsive element binding protein 1, cAMP-response element-binding protein-1, transactivator protein
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified antibody
tipo de anticuerpo
primary antibodies
clon
634-2, monoclonal
reactividad de especies
rat, mouse
fabricante / nombre comercial
Upstate®
técnicas
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable
isotipo
IgG1κ
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
dry ice
modificación del objetivo postraduccional
phosphorylation (pSer133)
Información sobre el gen
human ... CREB1(1385)
Descripción general
CREB is a β ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. Additionally, CREB signaling is involved in learning and memory in several organisms. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2.
Especificidad
Broad species cross-reactivity expected, based on sequence homology.
Phospho-CREB (Ser133).
Inmunógeno
A proprietary immunogen based on the amino acid sequence containing phospho-serine corresponding to residue 133 of human CREB.
Epitope: Ser133
Aplicación
Beadlyte Specificity Assay:
1:500 to 1:32,000 dilutions of this lot detected CREB peptide containing phosphoserine 133. The antibody did NOT recognize the nonphosphorylated peptide of the same sequence
Immunofluorescence / Immunocytochemistry:
This antibody has been reported by an independent laboratory to detect phospho-CREB (Ser133) using immunofluorescence.
1:500 to 1:32,000 dilutions of this lot detected CREB peptide containing phosphoserine 133. The antibody did NOT recognize the nonphosphorylated peptide of the same sequence
Immunofluorescence / Immunocytochemistry:
This antibody has been reported by an independent laboratory to detect phospho-CREB (Ser133) using immunofluorescence.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Use Anti-phospho-CREB (Ser133) Antibody, clone 634-2 (mouse monoclonal antibody) validated in ICC, IF, WB to detect phospho-CREB (Ser133) also known as active transcription factor CREB, cAMP responsive element binding protein 1.
Calidad
Routinely evaluated by western blot on RIPA lysates from forskolin treated rat PC-12 and mouse 3T3/A31 cells.
Western Blot Analysis:
A 1:1,000 to 1:4,000 dilution of this lot detected phospho-CREB (Ser 133) in RIPA lysates from forskolin treated rat PC-12 and mouse 3T3/A31 cells (Figure A).
Western Blot Analysis:
A 1:1,000 to 1:4,000 dilution of this lot detected phospho-CREB (Ser 133) in RIPA lysates from forskolin treated rat PC-12 and mouse 3T3/A31 cells (Figure A).
Descripción de destino
43 kDa
Forma física
Format: Purified
Protein G Purified
Purified mouse IgG1κ in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol. Liquid at -20ºC.
Almacenamiento y estabilidad
Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Nota de análisis
Control
Human breast carcinoma, forskolin- and FGF-treated SK-N-MC cell extracts.
Human breast carcinoma, forskolin- and FGF-treated SK-N-MC cell extracts.
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Opcional
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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