05-777-AF647

Anti-Phosphotyrosine Antibody, recombinant clone 4G10^® Antibody, Alexa Fluor^™ 647

clone 4G10, from mouse, ALEXA FLUOR^™ 647

• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• conjugado: ALEXA FLUOR^™ 647
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 4G10, monoclonal
• reactividad de especies (predicha por homología): all
• técnicas: immunocytochemistry: suitable
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: phosphorylation (pTyr)

Descripción

Descripción general

Phosphorylation plays an important role in regulating protein activities and various cellular signaling events. Posttranslational phosphorylation can occur on histidine (pHis), serine (pSer), threonine (pThr), and tyrosine (pTyr) residues. Due to the early advent of anti-pTyr antibodies, tyrosine phosphorylation remains the mostly studied signaling events in all biological research fields. Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. Before the availability of anti-pTyr antibodies, protein tyrosine phosphorylation was routiniely detected by time-consuming radioactive labeling. Although radioactive labeling is still considered as more sensitive, advancement in detection technology has greatly improved the detection limits of antibody-based methods. Anti-pTyr antibodies are commonly used in ELISA, immunoprecipitation, immunoblotting (including dot blot and Western blot), as well as indirect immunofluourescent detection of tyrosine phosphorylation in tissue and cell samples.

Especificidad

Recognizes tyrosine-phosphorylated proteins from all species.

Target modification is not species-specific

Inmunógeno

Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Purified via heavy chain C-terminus hexa-histidine tag by Nickel affinity matrices prior to conjugation with Alexa Fluor^™ 647.

Aplicación

Anti-Phosphotyrosine Antibody, clone 4G10, Alexa Fluor^™ 647 is a highly validated recombinant mouse monoclonal antibody that targets protein tyrosine phosphorylation and has been tested in Immunocytochemistry.

Research Category
Signaling

This recombinant monoclonal antibody is also available as HRP conjugate (Cat. No. 16-184), agarose conjugate (Cat. No. 16-199), biotin conjugate (Cat. No. 16-204), FITC conjugate (Cat. No. 16-205), PE conjugate (Cat. No. FCMAB323PE), as well as as unconjugated antibody (Cat. No. 05-777) for immunoaffinity purification, flow cytometry, immunocytochemistry, immunoprecipitation, and Western blotting applications.

Calidad

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected EGF-induced tyrosine phosphorylation of cellular proteins in A431 cells.

Descripción de destino

Variable depending on the size(s) of the tyrosine-phosphorylated protein(s).

Forma física

Protein G purified.

Purified mouse monoclonal IgG2a antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Información legal

4G10 is a registered trademark of Upstate Group, Inc.

ALEXA FLUOR is a trademark of Life Technologies

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable