05-690
Anti-HP1γ Antibody, clone 42s2
clone 42s2, Upstate®, from mouse
Sinónimos:
HP1 gamma, HP1 gamma homolog, Heterochromatin protein 1 homolog gamma, Modifier 2 protein, chromobox homolog 3, chromobox homolog 3 (Drosophila HP1 gamma), chromobox homolog 3 (HP1 gamma homolog, Drosophila), heterochromatin protein HP1 gamma, heterochro
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified antibody
tipo de anticuerpo
primary antibodies
clon
42s2, monoclonal
reactividad de especies
human, mouse
fabricante / nombre comercial
Upstate®
técnicas
ChIP: suitable
immunohistochemistry: suitable
western blot: suitable
isotipo
IgG1
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
dry ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... CBX3(11335)
Descripción general
Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication.
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated
Especificidad
Reactivity with other species has not been determined.
This antibody recognizes HP1γ protein, Mr ~25 kDa.
Inmunógeno
GST fusion protein corresponding to residues 17-173 of human HP1γ protein. Clone 42s2.
Aplicación
Immunohistochemistry:
This antibody has been reported by an independent laboratory to detect HP1γ positive cells in breast tissue.
Immunoprecipitation/Chromatin Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate HP1γ from nuclear extracts and formalin-cross-linked chromatin.
This antibody has been reported by an independent laboratory to detect HP1γ positive cells in breast tissue.
Immunoprecipitation/Chromatin Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate HP1γ from nuclear extracts and formalin-cross-linked chromatin.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Use Anti-HP1γ Antibody, clone 42s2 (Mouse Monoclonal Antibody) validated in ChIP, IHC, WB to detect HP1γ also known as Heterochromatin protein 1 homolog gamma.
Calidad
Routinely evaluated by western blot in acid-extracted HeLa cell lysates (Catalog #13-112).
Western Blot Analysis:
0.01-0.1 µg/mL of this lot detected HP1γ protein in acid-extracted HeLa cell lysates (Catalog # 13-112).
Western Blot Analysis:
0.01-0.1 µg/mL of this lot detected HP1γ protein in acid-extracted HeLa cell lysates (Catalog # 13-112).
Descripción de destino
25 kDa
Forma física
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1 in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175M NaCl, 0.07% sodium azide, and 30% glycerol. Liquid at -20ºC.
Almacenamiento y estabilidad
Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Nota de análisis
Control
HeLa acid extracts, HEK 293 nuclear extracts.
HeLa acid extracts, HEK 293 nuclear extracts.
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Due to license agreement restrictions, this product cannot be purchased for resale.
Información legal
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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