05-689

Anti-HP1α Antibody, clone15.19s2

clone 15.19s2, Upstate^®, from mouse

• Sinónimos: Antigen p25, HP1 alpha, HP1Hs alpha, Heterochromatin protein 1 homolog alpha, chromobox homolog 5 (Drosophila HP1 alpha), chromobox homolog 5 (HP1 alpha homolog, Drosophila), heterochromatin protein 1-alpha
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 15.19s2, monoclonal
• reactividad de especies: mouse, vertebrates, human
• fabricante / nombre comercial: Upstate^®
• técnicas: ChIP: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG1
• Nº de acceso NCBI: NM_012117
• Nº de acceso UniProt: P45973
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CBX5(23468)

Descripción

Descripción general

Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure. All three HP1 family members (α, β, and γ) are primarily associated with centromeric heterochromatin; however, HP1β and γ also localize to euchromatic sites in the genome. HP1 proteins are approximately 25 kDa in size and each contains a conserved amino-terminal chromodomain, followed by a variable hinge region and a conserved carboxy-terminal chromoshadow domain. The chromodomain facilitates binding to histone H3 tri-methylated on Lys9, a histone "mark" closely associated with centromeric heterochromatin. The variable hinge region binds both RNA and DNA in a sequence-independent manner. The chromoshadow domain mediates the dimerization of HP1 proteins, in addition to binding multiple proteins implicated in gene silencing and heterochromatin formation, including the SUV39H histone methyltransferase, the DNMT1 and DNMT3a DNA methyltransferases and the p150 subunit of chromatin-assembly factor-1 (CAF1). In addition to contributing to heterochromatin formation and propagation, HP1 and SUV39H are also found complexed with retinoblastoma (Rb) and E2F6 proteins, both of which function to repress euchromatic gene transcription in quiescent cells. HP1 proteins are subject to multiple types of post-translational modifications, including phosphorylation, acetylation, methylation, ubiquitination and sumoylation, suggesting multiple means of regulation.

Especificidad

This antibody recognizes HP1α

Inmunógeno

GST fusion protein corresponding to residues 2-191 of human HP1α protein.

Aplicación

Anti-HP1α Antibody, clone15.19s2 is a Mouse Monoclonal Antibody for detection of HP1α also known as HP1Hs alpha, Heterochromatin protein 1 homolog alpha & has been validated in ChIP, ICC, IHC, IP & WB.

Immunohistochemistry:
A previous lot of this antibody has been reported by an independent laboratory to detect HP1a in NIH/3T3 nuclei (Ayyanathan, K., 2003).

Immunoprecipitation/Chromatin Immunoprecipitation:
A previous lot of this antibody has been reported by an independent laboratory to immunoprecipitate HP1α from nuclear extracts and formalin-cross-linked chromatin (Ayyanathan, K., 2003).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

RNA Binding Protein (RBP)

Calidad

Routinely evaluated by western blot in acid-extracted HeLa cell lysates.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected HP1α protein in acid-extracted HeLa cell lysates.

Descripción de destino

29 kDa

Ligadura / enlace

Replaces: MABE479

Forma física

Format: Purified

Protein G Chromatography

Purified mouse monoclonal IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Handling Recommendations:


Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análisis

Control
HeLa, NIH/3T3 and COS cell lysates.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Due to license agreement restrictions, this product cannot be purchased for resale.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable