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Key Documents

05-598

Sigma-Aldrich

Anti-phospho-Histone H3 (Ser10) Antibody, clone RR002

clone RR002, Upstate®, from mouse

Sinónimos:

H3 histone, family 3A, H3S10P, Histone H3 (phospho S10), H3 histone, family 3A, H3 histone, family 3B, H3 histone, family 3B (H3.3B)

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

RR002, monoclonal

reactividad de especies

vertebrates, nematode, Caenorhabditis elegans, human

fabricante / nombre comercial

Upstate®

técnicas

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotipo

IgG

idoneidad

not suitable for ELISA applications

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

phosphorylation (pSer10)

Información sobre el gen

human ... HIST1H3F(8968)

Descripción general

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Especificidad

Broad species cross-reactivity expected due to sequence conservation.
This antibody recognizes Histone H3 phosphorylated at Serine 10.

Inmunógeno

Peptide (ARK[pS]TGGKAPRKQL-C) corresponding to amino acids 7-20 of human histone H3 with a c-terminal cyteine added for conjugation purposes.

Aplicación

Anti-phospho-Histone H3 (Ser10) Antibody, clone RR002 is a Mouse Monoclonal Antibody for detection of Histone H3 phosphorylated at serine 10. This purified mAb, also known as Anti-H3S10p, is published in peer reviewed journals and is validated in ICC, IHC, WB.
ELISA Analysis:
This antibody showed no immuno-reactivity with an unphosphorylated Histone H3 peptide (residues 7-20), nor with other histone phosphopeptides.

Immunohistochemistry:
A 1:1000 dilution of this antibody detected mitotic cells in C. elegans embryo sections as shown by an independent laboratory.

Immunocytochemistry:
This lot showed positive staining of mitotic cells in cultures of HeLa cells undergoing exponential growth

Calidad

Routinely evaluated by western blot on acid extracted proteins from colcemid-arrested HeLa cells.

Western Blot Analysis:
0.005-0.025 μg/mL of this lot detected phosphorylated Histone H3 in acid extracted proteins from colcemid-arrested HeLa cells.

Descripción de destino

17 kDa

Forma física

Format: Purified
Purified mouse IgG in buffer containing 0.1M Tris Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.

Nota de análisis

Control
UV-treated 293 cell extracts, UV-treated HeLa cell extracts, breast cancer tissue, HEPG2 cell extrats.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Nowak, S. J., et al
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