05-577
Anti-PP2A Antibody, C subunit, demethylated, clone 4b7
ascites fluid, clone 4b7, Upstate®
Sinónimos:
Anti-NEDLBA, Anti-PP2Ac, Anti-PP2CA, Anti-PP2Calpha, Anti-RP-C
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
ascites fluid
tipo de anticuerpo
primary antibodies
clon
4b7, monoclonal
reactividad de especies
vertebrates
fabricante / nombre comercial
Upstate®
técnicas
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotipo
IgG
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... PPP2CA(5515)
Especificidad
unmethylated PP2A, C subunit
Inmunógeno
peptide (C-RGEPHVTRRTPDYFL) corresponding to amino acids 295-309 of human PP2A, C subunit
Aplicación
Anti-PP2A Antibody, C subunit, demethylated, clone 4b7 is an antibody against PP2A for use in IC, IP & WB.
Research Category
Signaling
Neuroscience
Signaling
Neuroscience
Research Sub Category
Kinases & Phosphatases
Neurodegenerative Diseases
Kinases & Phosphatases
Neurodegenerative Diseases
Calidad
routinely evaluated by immunoblot on RIPA lysates from A431 cells
Descripción de destino
36kDa
Forma física
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Ascites
Almacenamiento y estabilidad
2 years at -20°C
Nota de análisis
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Información legal
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Juyeon Hwang et al.
The Journal of biological chemistry, 291(40), 21008-21019 (2016-08-11)
The protein phosphatase 2A (PP2A) subfamily of phosphatases, PP2A, PP4, and PP6, are multifunctional serine/threonine protein phosphatases involved in many cellular processes. Carboxyl methylation of the PP2A catalytic subunit (PP2Ac) C-terminal leucine is regulated by the opposing activities of leucine
Xiaoyu Xia et al.
Cell cycle (Georgetown, Tex.), 14(12), 1938-1947 (2015-04-04)
Leucine carboxyl methyltransferase-1 (LCMT1) and protein phosphatase methylesterase-1 (PME-1) are essential enzymes that regulate the methylation of the protein phosphatase 2A catalytic subunit (PP2AC). LCMT1 and PME-1 have been linked to the regulation of cell growth and proliferation, but the
Vitali Stanevich et al.
Molecular cell, 41(3), 331-342 (2011-02-05)
Proper formation of protein phosphatase 2A (PP2A) holoenzymes is essential for the fitness of all eukaryotic cells. Carboxyl methylation of the PP2A catalytic subunit plays a critical role in regulating holoenzyme assembly; methylation is catalyzed by PP2A-specific methyltransferase LCMT-1, an
Ying Zhou et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(12), 16414-16431 (2020-10-19)
Polyphyllin I (PPI) is a natural phytochemical drug isolated from plants which can inhibit the proliferation of cancer cells. One of the PPI tumor-inhibitory effects is through downregulating the expression of Cancerous Inhibitor of PP2A (CIP2A), the latter, is found
X X Yu et al.
Molecular biology of the cell, 12(1), 185-199 (2001-02-13)
Binding of different regulatory subunits and methylation of the catalytic (C) subunit carboxy-terminal leucine 309 are two important mechanisms by which protein phosphatase 2A (PP2A) can be regulated. In this study, both genetic and biochemical approaches were used to investigate
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