05-184
Anti-Src Antibody, clone GD11
clone GD11, Upstate®, from mouse
Sinónimos:
Anti-ASV, Anti-SRC1, Anti-THC6, Anti-c-SRC, Anti-p60-Src
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
mouse
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
GD11, monoclonal
reactividad de especies
avian, mink, mouse, rat, human
fabricante / nombre comercial
Upstate®
técnicas
immunoprecipitation (IP): suitable
western blot: suitable
isotipo
IgG1
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
dry ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... SRC(6714)
Descripción general
Src is a family of proto-oncogenic tyrosine kinases originally discovered by J. Michael Bishop and Harold E. Varmus. The discovery of Src family proteins has been instrumental to the modern understanding of cancer as a disease where normally healthy cellular signalling has gone awry.
Some tumor-inducing retroviruses (such as Rous sarcoma virus (RSV)), contain a gene called v-src (viral-sarcoma). It was found that the v-src gene in RSV is required for the formation of cancer.
Src tyrosine kinases transmit integrin-dependent signals central to cell movement and proliferation. Hallmarks of v-src induced transformation are rounding of the cell and the formation of actin rich podosomes on the basal surface of the cell. These structures are correlated with increased invasiveness, a process thought to be essential for metastasis.
v-src lacks the C-terminal inhibitory phosphorylation site, and is therefore constitutively active as opposed to normal src (c-src) which is only activated under certain circumstances where it is required (e.g. growth factor signaling). v-src is therefore an instructive example of an oncogene whereas c-src is a proto-oncogene.
Some tumor-inducing retroviruses (such as Rous sarcoma virus (RSV)), contain a gene called v-src (viral-sarcoma). It was found that the v-src gene in RSV is required for the formation of cancer.
Src tyrosine kinases transmit integrin-dependent signals central to cell movement and proliferation. Hallmarks of v-src induced transformation are rounding of the cell and the formation of actin rich podosomes on the basal surface of the cell. These structures are correlated with increased invasiveness, a process thought to be essential for metastasis.
v-src lacks the C-terminal inhibitory phosphorylation site, and is therefore constitutively active as opposed to normal src (c-src) which is only activated under certain circumstances where it is required (e.g. growth factor signaling). v-src is therefore an instructive example of an oncogene whereas c-src is a proto-oncogene.
Especificidad
Recognizes pp60src.
Inmunógeno
Epitope: amino acids 82-169
Recombinant chicken pp60src; v-src
Aplicación
Anti-Src Antibody, clone GD11 detects level of Src & has been published & validated for use in IP & WB.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Calidad
routinely evaluated on RIPA lysates from non-stimulated A431 carcinoma cells, RIPA lysates from EGF-stimulated A431 cells, mouse 3T3/A31 cells or Cos-1 cells transfected with pUSE Mouse src
Descripción de destino
60 kDa
Forma física
Format: Purified
Protein G Purified
Protein G Purified immunoglobulin in Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Almacenamiento y estabilidad
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Nota de análisis
Control
Positive Control Included: Non-Stim A431 Cell Lysate (12-301). Chicken Embryo Fibroblast (CEF) cells expressing Src protein and plated on fibronectin or Non-stimulated human A431 cell lysate
Positive Control Included: Non-Stim A431 Cell Lysate (12-301). Chicken Embryo Fibroblast (CEF) cells expressing Src protein and plated on fibronectin or Non-stimulated human A431 cell lysate
Información legal
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 2
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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