05-166

Anti-IGF-II Antibody, clone S1F2

clone S1F2, Upstate^®, from mouse

• Sinónimos: Anti-C11orf43, Anti-GRDF, Anti-IGF-II, Anti-PP9974, Anti-SRS3
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: S1F2, monoclonal
• reactividad de especies: human, rat
• fabricante / nombre comercial: Upstate^®
• técnicas: ELISA: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; neutralization: suitable; western blot: suitable
• isotipo: IgG1
• Nº de acceso NCBI: NM_000612
• Nº de acceso UniProt: P01344
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... IGF2(3481)

Descripción

Descripción general

Human Insulin-like Growth Factor II (IGFII) contains 67 amino acids and shares similar structural features with IGFI, including a 62% sequence homology. In human plasma, IGFI and IGFII are associated with IGF-binding proteins that transport the polypeptides and partially regulate their actions in vivo. In addition to the insulin receptor, IGFII binds to two forms of IGF receptors, both of which are widely distributed in different tissues and cultured cells. IGFII is mitogenic for a variety of cultured cells, including mouse 3T3 cells,12 normal rat kidney cells, human or chicken fibroblasts and MCF-7 human breast carcinoma cells.

Especificidad

Less than 10% cross-reactivity to human IGF-I (Tanaka, H, 1989; Suzuki, T, 1989).

This antibody detects IGF-II.

Inmunógeno

Partially purified rat IGF-II. Clone S1F2

Aplicación

Detect IGF-II using this Anti-IGF-II Antibody, clone S1F2 validated for use in ELISA, IP, IC, NEUT & WB.

Neutralization: 10 μg/mL of a previous lot inhibited the stimulatory effects of IGF-II (10 ng/mL) on DNA synthesis in chicken embryo fibroblasts. Cell viability was assessed using an ATP endpoint assay (ATPLiteÔ-M, Packard Instruments). Previous lots of antibody were tested to inhibit IGF-II-dependent incorporation of [3H]-thymidine

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

Calidad

Evaluated by Western Blotting on HeLa acid extracted nuclear preps (positive) and recombinant Histone H3 (negative).

Western Blotting Analysis:
1:5,000 – 1:10,000 dilution of this antibody was used to detect trimethylated-Histone H3 (Lys4) in HeLa nuclear acid extracts.

Descripción de destino

~7 kDa

Forma física

Ammonium sulfate precipitation and DEAE-cellulose chromatography

Format: Purified

Purified mouse monoclonal IgG1 in buffer containing PBS, pH 7.4., purified by ammonium sulfate precipitation and DEAE-cellulose chromatography. Frozen solution.

Almacenamiento y estabilidad

Stable for 1 year at -20ºC from date of receipt.

Nota de análisis

Control
Human head and neck tumours, angioma tissues

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable