05-1100X

Anti-Sulfotyrosine Antibody, Clone Sulfo-1C-A2

clone Sulfo-1C-A2, Upstate^®, from mouse

• Sinónimos: Clone Sulfo-1C-A2 Antibody, Sulfotyrosine Detection Antibody
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: culture supernatant
• tipo de anticuerpo: primary antibodies
• clon: Sulfo-1C-A2, monoclonal
• reactividad de especies: human, porcine, mouse, bovine
• reactividad de especies (predicha por homología): all
• fabricante / nombre comercial: Upstate^®
• técnicas: ELISA: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG2aκ
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

Sulfotyrosine is a common post-translational modification of secretory, plasma membrane, and lysosomal proteins. The sulfation reaction is catalyzed by specific sulfotransferase enzymes in the trans-Golgi-network. Tyrosine sulfation occurs in all multicellular organisms and up to 1% of the tyrosines in the total protein content of a cell can be sulfated. Sulfotyrosines mediates many receptor-ligand binding interactions, including the interaction of P-selectin glycoprotein ligand-1 with P-selectin, factor VIII binding to von Willebrand factor, and interactions between thrombin and hirudin. Sulfotyrosine is also involved in the entry of several parasites and viruses; for example, tyrosine sulfation of the HIV-1 co-receptor CCR5 is required for viral entry into host cells, and tyrosine sulfation of the Duffy antigen/receptor for chemokines is crucial for erythrocyte invasion by the malaria parasite Plasmodium vivax.
This clone was derived from the antibody described in Kehoe et al., 2006.

Especificidad

Recognizes sulfated tyrosine residues on various proteins. Does not recognize phosphotyrosine or unmodified tyrosines.

The antibody is predicted to recognize any sulfotyrosine, regardless of surrounding amino acid sequence.

Inmunógeno

Epitope: Sulfated Tyrosine

This antibody was developed using a phage display library as described in Kehoe et al., 2006. The selection antigens were KAKISDP-DY(SO3)MTGYMDAC and KDKKYATEY(SO3)-EYLDYDFC

Aplicación

Anti-Sulfotyrosine Antibody, Clone Sulfo-1C-A2 is a high quality Mouse Monoclonal Antibody for the detection of Sulfotyrosine & has been validated in WB, IP, ELISA.

Research Category
Signaling

Research Sub Category
General Post-translation Modification

WB, IP, ELISA

Calidad

Assayed using sulfatase treated bovine fibrinogen vs. untreated fibrinogen.

Descripción de destino

n/a

Forma física

Culture supernatant, purified by protein G affinity chromatography

Format: Purified

Mouse IgG2aκ purified supernatant in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4 .

Almacenamiento y estabilidad

Stable for 1 year from date of receipt when stored at +2-8°C.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable