05-615

Anti-SUV39H1 Antibody, clone MG44

ascites fluid, clone MG44, Upstate^®

• Sinónimos: KMT1A
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: ascites fluid
• tipo de anticuerpo: primary antibodies
• clon: MG44, monoclonal
• reactividad de especies: mouse, human
• fabricante / nombre comercial: Upstate^®
• técnicas: ChIP: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG
• Nº de acceso NCBI: NM_003173.2
• Nº de acceso UniProt: O43463
• Condiciones de envío: dry ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... SUV39H1(6839)

Descripción

Descripción general

Post -translational modification of histones, such as phosphorylation, acetylation, and methylation, are crucial for chromatin remodeling. SUV39H1 and Suv39h1 are the human and mouse homologs of Drosophila Su(var)3-9 protein, respectively. These proteins selectively methylate histone H3 at lysine 9 creating a high-affinity binding site for the HP1 proteins (heterochromatin protein 1). HP1 proteins are known to interact with transcription suppressor proteins, suggesting that the interactions with SUV39H1 and methylated histone H3 mediate a silence effect on the transcription of target genes. Furthermore, over-expression of SUV39H1 in HeLa cells causes a redistribution of endogenous HP1 proteins and growth retardation, suggesting that SUV39H1-mediated modulation of heterochromatin can impair cell cycle progression.

Especificidad

Recognizes SUV39H1.

Inmunógeno

MBP fusion protein corresponding to residues 1-412 of human SUV39H1

Aplicación

Anti-SUV39H1 Antibody, clone MG44 is a Mouse Monoclonal Antibody for detection of SUV39H1 also known as KMT1A, Histone H3-K9 Methyltransferase 1 & has been validated in ChIP, IP & WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Chromatin Biology

Calidad

routinely evaluated by immunoblot on U2OS cells lysed in RIPA with 0.1% SDS and sonicated

Descripción de destino

50 kDa

Ligadura / enlace

Replaces: MABE552

Forma física

Protein G Purified immunoglobulin in Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.

Unpurified

Almacenamiento y estabilidad

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Nota de análisis

Control
U2OS cell lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1