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04-902

Sigma-Aldrich

Anti-HA Tag Antibody, clone DW2, rabbit monoclonal

culture supernatant, clone DW2, from rabbit

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.43

origen biológico

rabbit

Nivel de calidad

forma del anticuerpo

culture supernatant

tipo de anticuerpo

primary antibodies

clon

DW2, monoclonal

reactividad de especies

vertebrates (common species)

técnicas

western blot: suitable

isotipo

IgG

Condiciones de envío

dry ice

modificación del objetivo postraduccional

unmodified

Descripción general

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule that corresponds to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Especificidad

Reactivity with other species has not been confirmed.
Recognizes recombinant proteins containing the HA epitope tag. The apparent molecular weight is related to the tagged protein.

Inmunógeno

KLH-conjugated, synthetic peptide containing the sequence …CYPYDVPDY… derived from the influenza virus hemaglutinin (HA) protein.

Aplicación

Anti-HA Tag Antibody, clone DW2 is an antibody against HA Tag for use in WB.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Western Blot Analysis: A 1:1,000-1:2,000 dilution of this lot detected HA-tagged WNT-1 protein expressed in HEK293 cells.

Calidad

Western Blot Analysis
Lysates from non-transfected (lane 1) and HAtagged WNT-1 transfected (lane 2) HEK293 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HA-Tag, clone DW2 (1:1,000 dilution).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Arrow indicates the HA-tagged protein (~49 kDa). The apparent molecular weight is related to the tagged protein.

Descripción de destino

Various

Ligadura / enlace

Replaces: 05-902

Forma física

Cultured supernatant in 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at -20°C from date of shipment. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análisis

Control
HA-tagged WNT-1 protein.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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