04-886

Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone A11W, rabbit monoclonal

culture supernatant, clone A11W, from rabbit

• Sinónimos: signal transducer and activator of transcription 5A, signal transducer and activator of transcription 5B, transcription factor STAT5B
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: culture supernatant
• tipo de anticuerpo: primary antibodies
• clon: A11W, monoclonal
• reactividad de especies: rat, chimpanzee, human, mouse, bovine
• técnicas: western blot: suitable
• isotipo: IgG
• idoneidad: not suitable for immunoprecipitation
• Nº de acceso NCBI: NM_003152; NM_012448
• Nº de acceso UniProt: P42229
• Condiciones de envío: dry ice
• modificación del objetivo postraduccional: phosphorylation (pTyr694/pTyr699)
• Información sobre el gen: bovine ... Stat5A(282375)

Descripción

Descripción general

The STAT proteins (signal transducer and activator of transcription) comprise a family of transcription factors. STATs are activated through tyrosine phosphorylation, mainly by JAK kinases, which results in their dimerization, nuclear translocation and regulation of gene expression. STAT5 activity is induced by numerous cytokines and growth factors. While STAT5A and STAT5B share 96% amino acid homology, the two isoforms have distinct physiological functions. Phosphorylation of tyrosine 694/699 is a key marker of STAT5A/B activation.

Especificidad

Phospho-STAT5A/B (Tyr694/699).

Inmunógeno

Epitope: pTyr694/699

KLH-conjugated, synthetic peptide containing the sequence DGpYVK in which pY corresponds to phospho-tyrosine at residue 694 of human STAT5A or residue 699 of STAT5B.

Aplicación

Detect phospho-STAT5A/B (Tyr694/699) using this Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone A11W validated for use in WB.

Research Category
Signaling

Apoptosis & Cancer

Research Sub Category
Transcription Factors

Calidad

Evaluated by western blot on RIPA lysates from 3T3/NIH cells untreated and treated with PDGF.

Western Blot Analysis:
A 1:1,000-1:2,000 dilution of this antibody detected phospho-STAT5A/B (Tyr694/699) in lysates from 3T3/NIH cells treated with PDGF.

Descripción de destino

~95 kDa

Ligadura / enlace

Replaces: 05-886

Forma física

Cultured supernatant containing 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análisis

Control
PDGF treated 3T3/NIH cells

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable