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608041

Sigma-Aldrich

L-Lysine-13C6,15N2 hydrochloride

99 atom % 13C, 99 atom % 15N, 95% (CP)

Sinónimos:

SILAC Amino Acid, (S)-2,6-Diaminohexanoic acid-13C6,15N2 hydrochloride, 13C and 15N Labeled Lys, 13C and 15N Labeled lysine hydrochloride

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About This Item

Fórmula lineal:
H215N(13CH2)413CH(15NH2)13CO2H · HCl
Número de CAS:
1200447-00-2
Peso molecular:
190.59
Número MDL:
MFCD00144647
Código UNSPSC:
12352209
ID de la sustancia en PubChem:
24881987
NACRES:
NA.12

pureza isotópica

99 atom % 13C
99 atom % 15N

Nivel de calidad

200

Análisis

95% (CP)

formulario

solid

actividad óptica

[α]25/D +20.7°, c = 2 in 5 M HCl

técnicas

bio NMR: suitable
protein expression: suitable

mp

263-264 °C (dec.) (lit.)

cambio de masa

M+8

cadena SMILES

Cl.[15NH2][13CH2][13CH2][13CH2][13CH2][13C@H]([15NH2])[13C](O)=O

InChI

1S/C6H14N2O2.ClH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H/t5-;/m0./s1/i1+1,2+1,3+1,4+1,5+1,6+1,7+1,8+1;

Clave InChI

BVHLGVCQOALMSV-GSMNGUNQSA-N

Descripción general

Lysine is a basic ketogenic amino acid with a protonated alkyl amino group. Lysine degradation results in the formation of acetoacetate. Acetylation/deacetylation of lysine residues in histones is a mechanism to regulate chromatin organization in eukaryotes. Isotope labeled amino acids are required for the production of isotopically labeled proteins.

Aplicación

  • L-Lysine-13C6,15N2 hydrochloride has been used in pSILAC (pulsed stable isotope labeling by amino acids in cell culture) mass spectrometry to study protein profile in cells transfected with microRNAs.
  • It has been used in SILAC isotopic labeling for the characterization of lysine racemase.
  • It has been used in SILAC labeling for phosphoproteomic measurements in neuro-2a cells treated with and without GFKP-19 (2-pyrrolidone derivative).
Pulsed Stable Isotope labeling of amino acid has been used to study host-cell function, survival, the precise intracellular pathways in protein synthesis of HIV-1 infected human monocytederived macrophages.

Envase

This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Cell-specific labeling enzymes for analysis of cell-cell communication in continuous co-culture.
Tape CJ, et al.
Molecular and Cellular Proteomics, 13, 1866-1876 (2014)
Whole cell proteome regulation by microRNAs captured in a pulsed SILAC mass spectrometry approach.
Ebner OA and Selbach M
Methods in Molecular Biology, 725, 315-331 (2011)
Deep Phosphoproteomic Measurements Pinpointing Drug Induced Protective Mechanisms in Neuronal Cells.
Yu C, et al.
Frontiers in Physiology, 7, 635-635 (2016)
Alex A Makarov et al.
Nature communications, 10(1), 3056-3056 (2019-07-13)
Lamin A is a nuclear intermediate filament protein critical for nuclear architecture and mechanics and mutated in a wide range of human diseases. Yet little is known about the molecular architecture of lamins and mechanisms of their assembly. Here we
Roberto Ravasio et al.
Science advances, 6(15), eaax2746-eaax2746 (2020-04-15)
The histone demethylase LSD1 is deregulated in several tumors, including leukemias, providing the rationale for the clinical use of LSD1 inhibitors. In acute promyelocytic leukemia (APL), pharmacological doses of retinoic acid (RA) induce differentiation of APL cells, triggering degradation of
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