Saltar al contenido
Merck

KSPRA2015

KiCqStart® Probe Assays

Predesigned probe assays for gene expression analysis

Sinónimos:

5’ nuclease assay, Hydrolysis Probes, Predesigned Probes, Probe-based Gene Expression, Probe-based RT-qPCR

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

Código UNSPSC:
41116133

temp. de almacenamiento

−20°C

Descripción general

Overview
Predesigned KiCqStart Probe Assays are for quantifying gene expression by RT-qPCR (Reverse Transcription Quantitative Real-Time PCR). To comply with MIQE, they have been developed using sophisticated bioinformatics tools and validated in silico to avoid off-target amplification1. KiCqStart Probe Assays are available as up to three sets of a forward primer, a reverse primer, and a Dual-Labeled Probe for genes from common model organisms.

1SNP loci have been avoided during the design process when possible. Please consult dbSNP to determine if your gene has unavoidable SNPs.

To learn more about KiCqStart Probe Assays, visit our Frequently Asked Questions section.

Specifications
Design characteristics
  • GC Content: 20 - 80%2
  • Melting Temperature: 58.0 °C2
  • Secondary Structure: None
  • Excessive 3′ Primer Clamping: Minimal
  • Amplicon size: 75 - 150 bases
  • Splice variant detected: Most prevalent

Oligo characteristics (primers)
  • Molecule: DNA (all sequences verified by mass spec QC)
  • Internal Modifications: None
  • Contents: 1 forward and 1 reverse primer
  • Length: 18 - 27 bases2
  • Purification: RP cartridge
  • Format: 3 OD minimum yield, dry; 2 tubes with 1 oligonucleotide each

Oligo characteristics (probe)
  • Molecule: DNA (all sequences verified by mass spec QC)
  • Internal Modifications: None
  • 5′ Reporter Dye: 6-FAM or HEX

Aplicación

Product use: gene expression analysis

Características y beneficios

KiCqStart® Probe Assays are sensitive, specific, and will save you time and money by eliminating the tedious steps required to develop an effective probe-based RT-qPCR Assay. The probe is labeled with Sigma′s dark Onyx Quencher (OQ), which emits heat instead of light and therefore offers an improved signal-to-noise ratio compared to traditional fluorescent quenchers.

Benefits of these assays include:
  • Target analysis is done
  • Design characteristics are set
  • Oligo characteristics are set
  • Easy to order

Propiedades físicas

Properties include:
  • Available species: human, mouse, and rat
  • Components: three oligos, one per tube
  • Shipping condition: dry, ambient temperature
  • Storage condition: dry or wet, single-use aliquots

Otras notas

Frequently Asked Questions section is available for this product.

Información legal

KiCqStart is a registered trademark of QIAGEN Beverly Inc.
OQ is a trademark of Sigma-Aldrich Co. LLC
Onyx Quencher is a trademark of Sigma-Aldrich Co. LLC

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documentos section.

Si necesita más asistencia, póngase en contacto con Atención al cliente

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico