KSPRA2015
KiCqStart® Probe Assays
Predesigned probe assays for gene expression analysis
Sinónimos:
5’ nuclease assay, Hydrolysis Probes, Predesigned Probes, Probe-based Gene Expression, Probe-based RT-qPCR
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About This Item
Código UNSPSC:
41116133
Productos recomendados
temp. de almacenamiento
−20°C
Descripción general
Overview
Predesigned KiCqStart Probe Assays are for quantifying gene expression by RT-qPCR (Reverse Transcription Quantitative Real-Time PCR). To comply with MIQE, they have been developed using sophisticated bioinformatics tools and validated in silico to avoid off-target amplification1. KiCqStart Probe Assays are available as up to three sets of a forward primer, a reverse primer, and a Dual-Labeled Probe for genes from common model organisms.
1SNP loci have been avoided during the design process when possible. Please consult dbSNP to determine if your gene has unavoidable SNPs.
To learn more about KiCqStart Probe Assays, visit our Frequently Asked Questions section.
Specifications
Design characteristics
Oligo characteristics (primers)
Oligo characteristics (probe)
Predesigned KiCqStart Probe Assays are for quantifying gene expression by RT-qPCR (Reverse Transcription Quantitative Real-Time PCR). To comply with MIQE, they have been developed using sophisticated bioinformatics tools and validated in silico to avoid off-target amplification1. KiCqStart Probe Assays are available as up to three sets of a forward primer, a reverse primer, and a Dual-Labeled Probe for genes from common model organisms.
1SNP loci have been avoided during the design process when possible. Please consult dbSNP to determine if your gene has unavoidable SNPs.
To learn more about KiCqStart Probe Assays, visit our Frequently Asked Questions section.
Specifications
Design characteristics
- GC Content: 20 - 80%2
- Melting Temperature: 58.0 °C2
- Secondary Structure: None
- Excessive 3′ Primer Clamping: Minimal
- Amplicon size: 75 - 150 bases
- Splice variant detected: Most prevalent
Oligo characteristics (primers)
- Molecule: DNA (all sequences verified by mass spec QC)
- Internal Modifications: None
- Contents: 1 forward and 1 reverse primer
- Length: 18 - 27 bases2
- Purification: RP cartridge
- Format: 3 OD minimum yield, dry; 2 tubes with 1 oligonucleotide each
Oligo characteristics (probe)
- Molecule: DNA (all sequences verified by mass spec QC)
- Internal Modifications: None
- 5′ Reporter Dye: 6-FAM or HEX
Aplicación
Product use: gene expression analysis
Características y beneficios
KiCqStart® Probe Assays are sensitive, specific, and will save you time and money by eliminating the tedious steps required to develop an effective probe-based RT-qPCR Assay. The probe is labeled with Sigma′s dark Onyx Quencher™ (OQ™), which emits heat instead of light and therefore offers an improved signal-to-noise ratio compared to traditional fluorescent quenchers.
Benefits of these assays include:
Benefits of these assays include:
- Target analysis is done
- Design characteristics are set
- Oligo characteristics are set
- Easy to order
Propiedades físicas
Properties include:
- Available species: human, mouse, and rat
- Components: three oligos, one per tube
- Shipping condition: dry, ambient temperature
- Storage condition: dry or wet, single-use aliquots
Otras notas
Frequently Asked Questions section is available for this product.
Información legal
KiCqStart is a registered trademark of QIAGEN Beverly Inc.
OQ is a trademark of Sigma-Aldrich Co. LLC
Onyx Quencher is a trademark of Sigma-Aldrich Co. LLC
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