G1007494

UHPLC Analysis of Nucleosides on Purospher^® STAR RP-18 endcapped

application for HPLC

Properties

• technique(s): HPLC: suitable
• test parameters: column: Hibar Purospher^® STAR RP-18 endcapped, 150 x 4.6 mm, 3 μm (1.50470.0001); mobile phase: [A] 30 mM ammonium phosphate, pH 5.3 with ammonium hydroxide in water; [B] Methanol; 97.5 [A] / 2.5 [B]; flow rate: 1.0 mL/min; pressure: 3060 psi 211 Bar; column temp.: 25 °C; detector: UV 254; injection: 5 μL; sample: 5 μg/mL of 12 nucleosides in 98:2 30mM ammonium phosphate pH 5.3 with ammonium hydroxide in water: methanol
• suitability: application for HPLC
• application(s): life science and biopharma

Description

Analysis Note

Nucleosides are structural subunits of nucleic acids, the macromolecules that convey genetic information in living cells. They contain a molecule of sugar (pentose) bonded to a nitrogen-containing organic ring compound. The nitrogen-containing compound is either a pyrimidine (cytosine, thymine, or uracil) or a purine (adenine or guanine). Nucleosides, derivatives and / or analogues can be used in many different industries and applications as in agrochemistry (herbicides, fungicides and insecticides), therapeutic drugs including a range of antiviral (HIV, hepatitis C virus and herpes simplex) and even antibiotics.

Legal Information

Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Pseudouridine
Cytidine
3-methylcytidine methosulfate
1-methyladenosine
Uridine
2-thiocytidine
5-methylcytidine
7-methylguanosine
2′-O-methylcytidine
Inosine
Guanosine
Ribothymidine
See All (12)