G005665
HPLC Analysis of Aminopterin and Related Compounds on Ascentis® Express C18
application for HPLC
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About This Item
technique(s)
HPLC: suitable
test parameters
column: Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U)
mobile phase: [A] 10 mM ammonium formate, pH 3.0; [B] acetonitrile; (90:10, A:B)
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: UV, 280 nm
injection: 5 μL
suitability
application for HPLC
application(s)
pharmaceutical (small molecule)
Analysis Note
This application demonstrates the suitability of the Ascentis C18 for the analysis of aminopterin, folic acid and methotrexate. Structures along with the optimized chromatogram obtained on the Ascentis Express C18 are presented
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Analyte
Description
Aminopterin
Folic acid
Methotrexate
standard
Product No.
Description
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