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59986-U

Supelco

Supel Carbon LC (2.7 µm) HPLC Columns

L × I.D. 10 cm × 2.1 mm, HPLC Column

Synonym(s):

Supel Carbon LC, 2.7 μm HPLC Column

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52

product name

Supel Carbon LC, 2.7 μm HPLC Column, column L × I.D. 10 cm × 2.1 mm

material

stainless steel column

Agency

suitable for USP L109

product line

Supel

packaging

1 ea of

parameter

≤250 °C temp. limit
≤9000 psi max. pressure (620 bar)

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

column L × I.D.

10 cm × 2.1 mm

surface area

155 m2/g

matrix

porous graphitic carbon (PGC) particle platform

particle size

2.7 μm

pore size

200 Å pore size

operating pH range

1-14

separation technique

mixed mode
reversed phase

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General description

Supel Carbon LC is a high-speed, ultra-high performance liquid chromatography column based on 200 Å porous graphitic carbon (PGC) particle technology. The PGC particle provides a 100% carbon plane for the analytes to interact with via hydrophobic interactions and through ionic interactions via the polar retention effect on graphite (PREG) effect. This particle design exhibits high column efficiency for polar compounds
and enhanced retention of compounds typically requiring hydrophilic interaction liquid chromatography (HILIC) to retain. Through the use of patent-pending synthetic procedures, a narrow particle size distribution is prevalent with this technology, further enhancing the chromatographic performance that this column can display.

Analysis Note

The target analytes include polar compounds, non-polar compounds, and structural isomers.

Recommended products

The use of a Supel Carbon LC 2.7 μm guard cartridge is recommended for the protection of your HPLC column. Guard cartridge kit, Cat. No. 59982-U, includes the appropriate Supel Carbon LC, 2.7 μm HPLC guard cartridge as well as the necessary holder. A pack of three replacement guard cartridges may be purchased using Cat. No. 59981-U. Additionally, a stand-alone guard cartridge holder may be purchased using Cat. No. 59999-U.

Legal Information

Supel is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Articles

Ultra-high performance liquid chromatographic (UHPLC) separation of 12 nucleosides using Supel™ Carbon LC column in 15 minutes for use in identification and quantitation of nucleoside biomarkers.

Ultra high-performance liquid chromatographic (UHPLC) separation of Vitamin D Metabolites― 3-epi-25-hydroxyvitamin D2 and 25-hydroxyvitamin D2, using Supel™ Carbon LC column with baseline separation, excellent peak shape, and sensitivity.

Ultra-high performance liquid chromatographic (UHPLC) separation of polar herbicides, paraquat and diquat using SupelTM Carbon LC column with baseline resolution, excellent peak shape, and good lot-to-lot reproducibility.

Ultra-high performance liquid chromatographic (UHPLC) separation of ephedrine and its three structural analogs based on polar retention effect on graphite (PREG) mechanism using Supel™ Carbon LC column.

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