ZRB1904

Anti-Fyn Antibody, clone 2F15 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2F15, recombinant monoclonal
• description: 2F15 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 60.76 kDa; observed mol wt ~56 kDa
• purified by: using Protein A
• species reactivity: rat, mouse, human
• species reactivity (predicted by homology): canine, porcine, bovine, monkey, feline
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal
• Protein ID accession no.: NP_001357458 and NP_694592
• UniProt accession no.: P06241
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... FYN(2534)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2F15 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Tyrosine-protein kinase Fyn. It targets an epitope within 20 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal region of human Tyrosine-protein kinase Fyn.

Application

Quality Control Testing

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Fyn in Jurkat cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Fyn in Ramos, C2C12, MOLT4, and PC12 cell lysates.

Flow Cytometry Analysis: 0.1 μg from a representative lot detected Fyn in one million Jurkat cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Fyn in human tonsil tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Fyn in Jurkat cells.

Affinity Binding Assay: A representative lot of this antibody bound Fyn with a KD of 3.6 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Tyrosine-protein kinase Fyn (UniProt: P06241; also known as EC:2.7.10.2, Proto-oncogene Syn, Proto-oncogene c-Fyn, Src-like kinase, SLK, p59-Fyn) is encoded by the FYN gene (Gene ID: 2534) in human. Fyn is a non-receptor tyrosine-protein kinase that plays a role in the regulation of cell growth and survival, cell adhesion, integrin-mediated signal transduction, cytoskeletal remodeling, cell motility, immune response, and axon guidance. It is present in lipid rafts and its palmitoylation is shown to be crucial for proper trafficking. Fyn plays a role in insulin sensitivity and lipid utilization by regulating LKB1 function and FYN knockout mice display significant reductions in adiposity, fasting glucose and insulin levels, and improved insulin sensitivity. FYN deletion improves plasma and tissue triglycerides, increases energy expenditure, and enhances fatty acid oxidation. Its protein kinase domain is localized in amino acids 271-534. It regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator Wiskott Aldrich syndrome (WAS) protein and the microtubule-associated proteins MAP2 and MAPT. Its phosphorylation on Tyr 531 in the C-terminal region by C-terminal Src kinase (CSK) maintains it an inactive state and its dephosphorylation by PTPRC/CD45 leads to its activation. It undergoes autophosphorylation at Tyr 420 and dephosphorylation at this site is shown to negatively regulate T- cell receptor signal transduction. Three isoforms of Fyn have been described that are produced by alternative splicing. Isoform 1 is highly expressed in the brain and isoform 2 is expressed in cells of hemopoietic lineages, especially T-lymphocytes. Isoform 3 lacks amino acids 233-287. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wiede, F., et al. (2011). J. Clin. Invest. 121(12); 4758-4774; Mustelin, T., et al. (1992). Eur. J. Immunol. 22(5); 1173-1178).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable