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Key Documents

T2319

Sigma-Aldrich

Trizma® hydrochloride solution

pH 7.5, BioPerformance Certified, 1 M, suitable for cell culture

Synonym(s):

Tris hydrochloride solution

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

grade

BioPerformance Certified
for molecular biology

Quality Level

200

form

solution

concentration

1 M

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, none detected
 bioburden, tested
 endotoxin, tested
≤5 ppm Heavy metals (as Pb)

pH

7.5

useful pH range

7.0-9.0

absorption

≤0.05 at 290 at 40%

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General description

A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Application

Trizma®hydrochloride solution has been used in Tween tris-buffered saline solution. It has also been used in the preparation of streptavidin-alkaline phosphatase used for enzyme linked immunosorbent assay (ELISA).

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The development of a sensitive and specific ELISA for mouse eosinophil peroxidase: assessment of eosinophil degranulation ex vivo and in models of human disease
Ochkur SI, et al.
Journal of Immunological Methods, 375(1-2), 138-147 (2012)
Genomic maps of long noncoding RNA occupancy reveal principles of RNA-chromatin interactions.
Ci C Chu, Kun K Qu, Franklin L FL Zhong, Steven E SE Artandi, Howard Y HY Chang
Molecular Cell, 44, 667-678 null
An in vitro assay for sonothrombolysis based on the spectrophotometric measurement of clot thickness
Wang Z, et al.
Journal of Ultrasound in Medicine : Official Journal of the American Institute of Ultrasound in Medicine, 36(4), 681-698 (2017)
Daniela Amann-Zalcenstein et al.
Nature immunology, 21(12), 1574-1584 (2020-10-21)
A classical view of blood cell development is that multipotent hematopoietic stem and progenitor cells (HSPCs) become lineage-restricted at defined stages. Lin-c-Kit+Sca-1+Flt3+ cells, termed lymphoid-primed multipotent progenitors (LMPPs), have lost megakaryocyte and erythroid potential but are heterogeneous in their fate.
Zuojun Wang et al.
Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine, 36(4), 681-698 (2017-02-06)
For improved thrombolysis therapy based on ultrasound irradiation, researchers and practitioners would strongly benefit from an easy and efficient in vitro assay system of thrombolysis activity involving irradiated ultrasound. For the present study, we designed a new in vitro sonothrombolysis
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