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Key Documents

SRP8045

Sigma-Aldrich

CD40L, Soluble mouse

recombinant, expressed in CHO cells, FLAG® tagged, >95% (SDS-PAGE)

Synonym(s):

ACRP30headless:CD154, ACRP30headless:CD40L, ACRP30headless:TNFSF5, ADIPOQ-CD40L

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

mouse

recombinant

expressed in CHO cells

tag

FLAG® tagged

Assay

>95% (SDS-PAGE)

form

lyophilized

mol wt

35-40 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

<0.01 EU/μg endotoxin, tested

UniProt accession no.

P27548

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Cd40lg(21947)

General description

CD40L (cluster of differentiation 40 ligand), a member of TNF (tumor necrosis factor) superfamily, is a membrane bound protein. This protein is predominantly expressed transiently by activated CD4+ T cells either as a cell-surface molecule or a secreted multimer protein. Activated B cells, certain DC (dendritic cell) subsets, platelets, and smooth muscle cells also express this protein.

Biochem/physiol Actions

CD40L (cluster of differentiation 40 ligand) interacts with its receptor CD40 which in turn helps mediate the interaction between APCs (antigen presenting cells) and lymphocytes. Activated platelets produce CD40L which then participates in multiple processes such as endothelial cell reactions, formation of germinal center, T-helper cell priming, and cytotoxic T-cell activation. Platelets are also involved in the pathogenesis of asthma through CD40L where they inhibit the production of regulatory T-cells and promote polarization of T-helper cells. Studies in mice show that this protein confers protection against influenza virus, though in a CD4+ T cell independent manner.

Physical form

Lyophilized from PBS.

Reconstitution

Reconstitute with 100 μL deionized water.

Other Notes

Mouse CD40L (aa 115-260) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Anwar M Hashem et al.
Journal of immunology (Baltimore, Md. : 1950), 193(2), 722-734 (2014-06-15)
CD40L, a key regulator of the immune system, was studied as both a targeting ligand and a molecular adjuvant in nucleoprotein (NP)-based host defense against influenza in mouse models with different genetic backgrounds. Adenoviral vectors secreting NP-CD40L fusion protein (denoted
Jun Tian et al.
Cellular & molecular immunology, 12(6), 700-707 (2014-11-25)
Platelet activation is associated with multiple immune responses and the pathogenesis of various immune-related diseases. However, the exact role and the underlying mechanism of platelets in the progression of allergic asthma remain largely unclear. In this study, we demonstrate that

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