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SRP5150

Sigma-Aldrich

Vimentin, His tagged human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

FLJ36605, VIM

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About This Item

CAS Number:
414913-88-5
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥90% (SDS-PAGE)

form

buffered aqueous glycerol solution

mol wt

~60 kDa

NCBI accession no.

NM_003380

application(s)

cell analysis

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... VIM(7431)

General description

Vimentin belongs to the intermediate filament family of proteins. It is a key structural component of eukaryotic cells and is associated with lymphocytes and neutrophils. Vimentin exists as a tripartite domain structure. It is expressed in mesenchymal cells. The vimentin gene is mapped to human chromosome 10p13.

Application

Vimentin, His tagged human has been used in the glass surface immobilization in anti-vimentin binding in force measurement by atomic force microscopy (AFM).

Biochem/physiol Actions

Vimentin (VIM) stabilizes cytoskeletal interactions and is crucial for the placement of organelles in the cytosol. It modulates apoptosis in lymphocytes and is involved in the pathogenesis of inflammatory diseases VIM may serve as a potential marker for sepsis diagnosis. It is also essential for lens integrity maintenance.
Vimentin is a member of intermediate filament family of proteins and is an important structural feature of eukaryotic cells. Along with microtubules and actin microfilaments, Vimentin make up the cytoskeleton component of cells. Studies have shown that Vimentin is attached to the nucleus, endoplasmic reticulum and mitochondria, either laterally or terminally. Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol. Thus, Vimentin plays a key role in maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions

Physical form

Supplied in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Pictograms

Exclamation markHealth hazard
GHS07,GHS08

Signal Word

Danger

Hazard Statements

H315 - H319 - H360D

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Vera L Costa et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 16(23), 5842-5851 (2010-10-27)
To identify a panel of epigenetic biomarkers for accurate bladder cancer (BlCa) detection in urine sediments. Gene expression microarray analysis of BlCa cell lines treated with 5-aza-2'-deoxycytidine and trichostatin A as well as 26 tissue samples was used to identify
T Katsumoto et al.
Biology of the cell, 68(2), 139-146 (1990-01-01)
The three-dimensional arrangement of vimentin intermediate filaments (IF) was studied in 3Y1, rat fibroblastic cell line, to elucidate its biological role in the cell. While actin filaments were observed exclusively in the superficial part of the cell, vimentin IF were
Longxiang Su et al.
Scientific reports, 9(1), 5747-5747 (2019-04-07)
New diagnostic biomarkers or therapeutic targets for sepsis have substantial significance for critical care medicine. In this study, 192 differentially expressed proteins were selected through iTRAQ. Based on cluster analysis of protein expression dynamics and protein-protein interactions, hemopexin, vimentin, and
Intermediate filaments: structure, dynamics, function, and disease.
E Fuchs et al.
Annual review of biochemistry, 63, 345-382 (1994-01-01)
R Kawamura et al.
Journal of nanobiotechnology, 14(1), 74-74 (2016-11-05)
The field of structural dynamics of cytoskeletons in living cells is gathering wide interest, since better understanding of cytoskeleton intracellular organization will provide us with not only insights into basic cell biology but may also enable development of new strategies

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