SRP0723
YSK4 (MAP3K19), Active
PRECISIO® Kinase, recombinant
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About This Item
UNSPSC Code:
12352202
Recommended Products
recombinant
expressed in baculovirus infected Sf9 cells
Assay
70-95% (SDS-PAGE)
form
frozen liquid
specific activity
30-45 nmol/min·mg (radiometric)
mol wt
~83 kDa
concentration
0.05 μg/μL
accession no.
NM_025052
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... YSK4(80122)
Application
Useful for kinase assay and Western blot.
Biochem/physiol Actions
Recombinant human YSK4 (MAP3K19) (941-end) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. YSK4 (MAP3K19, RCK) belongs to STE Ser/Thr protein kinase family and STE20 subfamily. The YSK4 gene is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, and rat (1). The important paralog is MAP3K1 gene. The RCK ligand-binding domains form an octameric gating ring (2) in the large-conductance Ca2+-activated K+ channels (3). The YSK4 kinase function has not been made clearly yet.
Reconstitution
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Storage and Stability
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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