SAB4200777

Anti-LbCas12a (Cpf1) antibody, Mouse monoclonal

clone LbCpf1, purified from hybridoma cell culture

• Synonym(s): Anti-CRISPR-associated endonuclease AsCas12a from Lachnospiraceae bacterium ND2006, Anti-LbCpf1 antibody, Mouse monoclonal
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: LbCpf1, monoclonal
• form: buffered aqueous solution
• mol wt: ~135 kDa
• concentration: ~1.0 mg/mL
• technique(s): immunoblotting: 0.6-1.2 μg/mL using human HEK-293T cells over-expressing LbCpf1 protein; immunofluorescence: 0.25-0.5 μg/mL using human HEK-293T cells over-expressing LbCpf1 protein; immunoprecipitation (IP): 1-2.5 μg/test using lysate of human HEK-293T cells over-expressing LbCpf1 protein
• isotype: IgG1
• UniProt accession no.: A0A182DWE3
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Clustered, regularly interspaced, short palindromic repeat (CRISPR) systems encode RNA-guided endonucleases that are essential for bacterial adaptive immunity. Depending on the architecture of the effector-CRISPR RNA (crRNA) interference module, different CRISPR-Cas systems could be assigned into two classes1: class 1 systems of multi-subunit complex, such as Cascade and class 2 systems of single enzyme, such as Cas9.
Cpf1 (CRISPR from Prevotella and Francisella 1) belongs to class 2 type V CRISPR-Cas endonuclease system. Cpf1 comprise several differences from Cas9 protein including cleavage with 5′ overhangs, a shorter guide RNA and a longer distance between the seed sequence and cleavage site.
LbCpf1, Cpf1 from Lachnospiraceae bacterium ND2006, was examined together with 15 members of Cpf1 nuclease family and proved to mediate efficient genome editing in HEK293FT cells with improved results compared to SpCas9.5 According to thr crystal structure, LbCpf1 has a triangle-shaped architecture with a large positively charged channel at the centre.7 The crRNA binding was shown to induce the pronounced structural rearrangements of LbCpf1, leading to formation of a substrate-binding conformation of LbCpf1. Over-expressed in plant cells, LbCpf1 demonstrated more than 10-fold transcriptional repression of the target gene.
Monoclonal anti-LbCpf1 antibody can provide a useful tool for genome editing research, such as detecting and monitoring LbCpf1 positively transfected cells.

Immunogen

Recombinant LbCpf1

Application

Monoclonal Anti-LbCpf1 specifically recognizes Cpf1 from Lachnospiraceae bacterium ND2006. The product may be used in several immunochemical techniques including Immunoblotting (∼135 kDa), Immunofluorescence and Immunoprecipitation. Monoclonal Anti-LbCpf1 does not cross react with Cpf1 from Acidaminococcus sp. (strain BV3L6) ), SpCas9 from Streptococcus pyogenes bacteria and FnCas9 from Francisella novicida bacteria.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.
In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable