SAB4200748
Monoclonal Anti-Human IgM (μ-chain specific)-Peroxidase antibody produced in mouse
clone MB-11, purified immunoglobulin
Synonym(s):
Anti-Human immunoglobulin M
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
Recommended Products
biological source
mouse
Quality Level
conjugate
peroxidase conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
MB-11, monoclonal
form
lyophilized powder
species reactivity
human
concentration
~2.0 mg/mL
technique(s)
ELISA: 1:40,000-1:80,000 using 2.5 μg/mL Human IgM for coating
isotype
IgG2b
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Monoclonal Anti-Human IgM (mouse IgG2b isotype) is derived from the MB-11 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with purified human IgM protein. Immunoglobulin M (IgM) is the major class of surface immunoglobulins on lymphocytes membranes. IgM has a pentameric structure, in which monomers are linked together via disulfide bonds. Surface IgM is expressed on immature and mature B cells, whereas IgM heavy chain is expressed intracellularly in pre-B cells. IgM serves as the antigen receptor of naive B cells.
Specificity
Monoclonal Anti-Human IgM (μ-chain) specifically recognizes μ chain of Human IgM. The antibody shows no cross-reactivity with human IgG, Fab, Fc, kappa, lambda and gamma chains.
Immunogen
purified human IgM protein
Application
Monoclonal Anti-Human IgM (μ-chain specific)-Peroxidase antibody produced in mouse may suitable for use in enzyme-linked immunosorbent assay (ELISA).
Biochem/physiol Actions
IgM is suggested to act as the first line of defense during microbial infections. Secreted IgM (sIgM) is involved in B cell maturation and complement activation. Detection of IgM antibodies in a patient′s serum may indicates a recent infection and IgM in a neonate′s serum may indicate on intrauterine infection (e.g. congenital rubella syndrome). In addition, the development of anti-donor IgM after combined liver-kidney transplantation has been shown to provide a graft- protecting effect. Anti-Human IgM (μ-specific) antibodies can be used in detection of the abnormal concentration of IgM in patient sera and IgM-associated pathologies, such as selective IgM immunodeficiency (SIgMD) and the hyperimmunoglobulin M (hyper-IgM or HIGM) syndromes.
Physical form
Supplied as a lyophilized powder
Storage and Stability
Store the lyophilized product at 2–8 °C. For extended storage after reconstitution, keep at –20 °C in working aliquots. Avoid repeated freeze-thaw cycles. For continuous use after reconstitution, keep at 2–8 °C for up to 1 month. Solutions at working dilution should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Natural antibodies: from first-line defense against pathogens to perpetual immune homeostasis
Maddur MS, et al.
Clinical Reviews in Allergy & Immunology, 58(2), 213-228 (2020)
Leman Yel et al.
International archives of allergy and immunology, 150(3), 291-298 (2009-06-06)
IgM deficiency is a dysgammaglobulinemia characterized by isolated low levels of serum IgM. Patients with IgM deficiency may exhibit various clinical manifestations. However, IgM deficiency still remains to be explored with regard to diagnosis and treatment. Through a retrospective chart
Chloe C McAlister et al.
Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society, 10(2), 315-319 (2004-02-06)
The mechanism by which a liver transplantation might protect a simultaneous kidney transplant in a crossmatch-positive recipient is unknown. Flow cytometry crossmatch (FCXM) has increased the sensitivity of donor-specific antibody (DSA) detection compared with complement-dependant cytotoxicity (CDC). Here we compare
Immunobiology: The Immune System in Health and Disease.
Charles A Janeway, Jr, Paul Travers, Mark Walport, and Mark J Shlomchik
Immunobiol., 5 (2001)
L D Notarangelo et al.
Immunodeficiency reviews, 3(2), 101-121 (1992-01-01)
Immunodeficiency with hyper-IgM (HIM) is a rare disorder characterized by recurrent infections associated with low IgG and IgA, and normal to increased IgM serum levels. Both primary and secondary forms of HIM syndrome have been reported. Among primary HIM syndrome
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