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SAB4200141

Anti-Rictor antibody produced in rabbit

Name: Anti-Rictor antibody produced in rabbit
Brand: SIGMA

~1-2 μg/mL, affinity isolated antibody

Synonym(s):

ANTI-Pianissimo, ANTI-Rapamycin-insensitive companion of mTOR, ANTI-TORC2-specific protein AVO3, Anti-RPTOR independent companion of MTOR, complex 2, AVO3

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.44

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

~190 kDa

species reactivity

human, rat, mouse

packaging

antibody small pack of 25 μL

concentration

~1-2 μg/mL

technique(s)

immunohistochemistry: 5-10 μg/mL using a working concentration of 5-10 ug/mL is recommended using heat-retrieved formalin-fixed, paraffin-embedded rat testis sections and biotin / ExtrAvidinPeroxidase staining system.
immunoprecipitation (IP): 5-10 μg using a working amount of 5-10 μg is recommended using lysates of human HeLa cells.
western blot: 1-2 μg/mL using a working concentration of 1-2 μg/mL is recommended using whole extracts of mouse C2C12 cells.

UniProt accession no.

Q6R327

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RICTOR(253260)
mouse ... rictor(78757)
rat ... rictor(310131)

General description

Rictor (rapamycin-insensitive companion of mTOR) is a subunit of the mammalian target of rapamycin complex 2 (mTORC2). The mTORC2 complex contains mTOR, mLST8, mSIN1, deptor, protor-1 and rictor. mTOR substrate that regulates cell size.

m-TOR is a conserved kinase that primarily regulates cell growth. Rictor is a rapamycin-insensitive companion of m-TOR and regulates the phosphorylation of Akt/PKB. Rictor also phosphorylates PKCα and actin cytoskeleton .

Specificity

Anti-Rictor antibody is specific for human, mouse and rat rictor. In immunoblotting, detection of the rictor band (approx. 190 kDa) is specifically inhibited by the immunizing peptide.

Immunogen

The corresponding sequence is identical in mouse and rat rictor.

Application

Anti-Rictor antibody is suitable for use in immunohistochemistry (5-10 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded rat testis sections and biotin/ExtrAvidin Peroxidase staining system). The product may also be used for immunoprecipitation (5-10 μg using lysates of human HeLa cells), and immunoblot (1-2 μg/mL is using whole extracts of mouse C2C12 cells).

Biochem/physiol Actions

Rictor (rapamycin-insensitive companion of mTOR) regulates cell survival and cytoskeletal organization through the regulation of Akt/ protein kinase B (PKB) and protein kinase C alpha (PKCα).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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mTORC2 activity is elevated in gliomas and promotes growth and cell motility via overexpression of rictor

Masri J, et al.

Cancer research, 67(24), 11712-11720 (2007)

Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex.

D D Sarbassov et al.

Science (New York, N.Y.), 307(5712), 1098-1101 (2005-02-19)

Deregulation of Akt/protein kinase B (PKB) is implicated in the pathogenesis of cancer and diabetes. Akt/PKB activation requires the phosphorylation of Thr308 in the activation loop by the phosphoinositide-dependent kinase 1 (PDK1) and Ser473 within the carboxyl-terminal hydrophobic motif by

Targeting mTORC2 inhibits colon cancer cell proliferation in vitro and tumor formation in vivo

Roulin D, et al.

Molecular Cancer, 9(1), 57-57 (2010)

Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton.

D D Sarbassov et al.

Current biology : CB, 14(14), 1296-1302 (2004-07-23)

The mammalian TOR (mTOR) pathway integrates nutrient- and growth factor-derived signals to regulate growth, the process whereby cells accumulate mass and increase in size. mTOR is a large protein kinase and the target of rapamycin, an immunosuppressant that also blocks

Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive.

Estela Jacinto et al.

Nature cell biology, 6(11), 1122-1128 (2004-10-07)

The target of rapamycin (TOR) is a highly conserved protein kinase and a central controller of cell growth. In budding yeast, TOR is found in structurally and functionally distinct protein complexes: TORC1 and TORC2. A mammalian counterpart of TORC1 (mTORC1)

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