S4019

Sodium bicarbonate

Hybri-Max^™, powder, suitable for hybridoma, ≥99.5%

• Synonym(s): Sodium hydrogen carbonate
• Linear Formula: NaHCO₃
• CAS Number: 144-55-8
• Molecular Weight: 84.01
• Beilstein: 4153970
• EC Number: 205-633-8
• MDL number: MFCD00003528
• UNSPSC Code: 12161702
• PubChem Substance ID: 57654616
• NACRES: NA.75

Properties

• grade: Hybri-Max^™
• Assay: ≥99.5%
• form: powder
• technique(s): cell culture | hybridoma: suitable
• impurities: endotoxin, tested
• pH: 8.3 (0.1 molfreshly prepared)
• pKa (25 °C): (1) 6.37, (2) 10.25 (carbonic acid)
• solubility: water: soluble 100 mg/mL
• density: 2.16 g/mL at 25 °C (lit.)
• SMILES string: [Na+].OC([O-])=O
• InChI: 1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1
• InChI key: UIIMBOGNXHQVGW-UHFFFAOYSA-M

Description

Application

Sodium bicarbonate has been used as a supplement:

• in minimum essential medium (MEM)/flushing medium for embryo isolation
• in TCM 199 medium for the collection of cumulus-oocyte complexes (COCs) and in vitro maturation (IVM)
• in IVM medium for in vitro maturation of COCs

Biochem/physiol Actions

Sodium bicarbonate occurs naturally as the mineral nahcolite. It is used to prepare many sodium salts as well as a carbon dioxide source. Sodium bicarbonate is used in the manufacture of fire extinguishers and cleaning agents. Bicarbonate plays an important role in pH regulation in vivo. In biochemistry, molecular biology, and chromatography, sodium bicarbonate is used as a buffer. This Hybri-Max^™ product is suitable for use in hybridoma and cell culture research (2.2 mg/ml).

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves