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RAB0350

Sigma-Aldrich

Cell-Based ELISA for detecting phospho-ERK1 / 2 (pThr202 / pTyr204) in cultured cell lines

96 assays

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

usage

96 assays

species reactivity

rat, human, mouse

technique(s)

ELISA: suitable
capture ELISA: suitable

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... MAPK1(5594) , MAPK3(5595)
mouse ... MAPK1(26413) , MAPK3(26417)
rat ... MAPK1(16590) , MAPK3(50689)

General description

The Cell-Based ERK1/2 (pThr202 / pTyr204) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of ERK1/2 (pThr202 / pTyr204) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human, mouse and rat cell lines.

Immunogen

ERK1/2 (pThr202 / pTyr204) synthetic phosphopeptide

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABBLOCK5x Blocking Solution (Item F)SDS
  • RABFIX1Fixing Solution (Item D)SDS
  • RABHRP1HRP-conjugated Anti-Rabbit IgG Concentrate (Item I1)SDS
  • RABHRP2HRP-conjugated Anti-Mouse IgG Concentrate (Item I2)SDS
  • RABPLATE1Uncoated MicroplateSDS
  • RABQUENCHQuenching Solution for Cell-based ELISA Assay (Item E)SDS
  • RABSTOP1Stop SolutionSDS
  • RABTMB1TMB Substrate Reagent (Item J)SDS
  • RABWASH120X Wash Buffer Concentrate ASDS
  • RABWASH220X Wash Buffer Concentrate BSDS
See All (10)

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Certificates of Analysis (COA)

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