R0260

BamH I from Bacillus amyloliquefaciens H

Restriction Enzyme

• CAS Number: 81295-09-2
• Enzyme Commission number: 3.1.21.4 (BRENDA, IUBMB)
• MDL number: MFCD00163008
• UNSPSC Code: 12352204

Properties

• grade: for molecular biology
• form: buffered aqueous glycerol solution
• concentration: 10,000 units/mL
• shipped in: wet ice
• storage temp.: −20°C

Description

Specificity

Recognition sequence: 5′-G/GATCC-3′
Cutting results: a 2-10-fold Bam HI overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 60 °C for 15 minutes.
Star activity: To prevent star activity, avoid suboptimal reaction conditions containing low salt concentration, high glycerol (>5%) and high pH 8.0.

Application

BamHI is a DNA restriction endonuclease that is used in molecular biology applications to cleave DNA at the recognition sequence 5′-G/GATCC-3′ to generate 5′-cohesive termini.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SB (B8781).

Physical form

Solution in 10 mM Tris-HCl, pH 7.4 , 1 mM EDTA, 1mM dithioerythritol, 300 mM KCl, 0.01% Polydocanol (v/v), 50% glycerol (v/v), at 4°C