P8913

Phospholipase A₂ from bovine pancreas

lyophilized powder, ≥20 units/mg protein

• Synonym(s): Lecithinase A, PLA₂, Phosphatidylcholine 2-acylhydrolase
• CAS Number: 9001-84-7
• Enzyme Commission number: 3.1.1.4 (BRENDA, IUBMB)
• EC Number: 232-637-7
• MDL number: MFCD00131890
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• form: lyophilized powder
• Quality Level: 200
• specific activity: ≥20 units/mg protein
• composition: Protein, 5-20% biuret
• UniProt accession no.: P00593
• storage temp.: −20°C
• Gene Information: cow ... PLA2G1B(282457)

Description

General description

Phospholipase A2 from bovine pancreas is a calcium-dependent lipolytic enzyme.

Application

Phospholipase A2 from bovine pancreas has been used to determine rat renal proximal tubular segments (PTS) viability during oxygenation and hypoxia-reoxygenation and to study the impact of phospholipase A2 on tubular cell triglyceride levels.

Biochem/physiol Actions

Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells.

Packaging

Package size based on protein content

Unit Definition

One unit will hydrolyze 1.0 μmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH 8.0 and 37 °C.

Physical form

Contains Tris buffer salts.

Analysis Note

Protein determined by biuret.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves