P6278
Peroxidase Inactivated
≤0.02 units/mg protein (biuret)
Synonym(s):
Peroxidase, inactivated from horseradish peroxidase
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About This Item
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form
powder
Quality Level
200
300
specific activity
≤0.02 units/mg protein (biuret)
impurities
salt, free
solubility
0.85% sodium chloride: soluble 15 mg/mL, clear to slightly hazy, colorless to light yellow
storage temp.
2-8°C
Related Categories
Application
Inactivated peroxidase is used to study regeneration of inactivated peroxidase enzymes. It has been used to study the mechanism of peroxidase solubilization and it is used to study antitumor activity of immobilized peroxidase systems. It may be used for developing differential staining techniques in nerve tissue preparations.
Biochem/physiol Actions
HRP readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors. The optimal pH is 6.0-6.5 and the enzyme is most stable in the pH range of 5.0-9.0. HRP can be conjugated to antibodies by several different methods that include the use of glutaraldehyde, periodate oxidation, disulfide bonds, and also via amino- and thiol-directed cross-linkers. It is smaller and more stable than the enzyme labels, β-galactosidase and alkaline phosphatase. Hence, it is the most desired label. Also, its glycosylation leads to lower non-specific binding. It is also used for the determination of glucose and peroxides in solution. Sodium azide, cyanide, L-cystine, dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and ions such as Cd2+, Co2+, Cu2+, Fe3+, Mn2+, Ni2+, and Pb2+ are found to inhibit the enzyme activity.
Inactivated peroxidase reacts with goat and rabbit anti-peroxidase. It may be reactivated various methods such as heating.
Unit Definition
One unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20°C.
Preparation Note
Prepared from Type VI horseradish peroxidase by a modification of the method of Teale.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Cleavage of the haem-protein link by acid methylethylketone.
F W TEALE
Biochimica et biophysica acta, 35, 543-543 (1959-10-01)
REGENERATION OF HEAT-INACTIVATED PEROXIDASE
Sigmund Schwimmer
The Journal of Biological Chemistry, 154, 487-495 (1944)
Antitumor Activity of an Immobilized Peroxidase System Against Murine Ehrlich Ascites Is Mediated by the Immune System
Steven H. Combs, Clinton D. Morgan, et al.
Exp. Biol. Med, 204, 306-311 (1993)
C J Forehand et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 2(5), 654-652 (1982-05-01)
This study of spinal cord injury in bullfrog (Rana catesbeiana) tadpoles using the neuroanatomical tracer horseradish peroxidase (HRP) was undertaken to determine (1) whether the same anatomical regions that normally give rise to ascending or descending spinal tracts do so
Dual projections of single neurons are visualized simultaneously: use of enzymatically inactive [3H]HRP.
N L Hayes et al.
Brain research, 165(2), 321-326 (1979-04-13)
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