N7782
Anti-Nitric Oxide Synthase, Inducible antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
Anti-iNOS
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About This Item
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biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 130 kDa
species reactivity
rat, mouse
technique(s)
microarray: suitable
western blot: 1:10,000 using cells extract of murine RAW 264.7 macrophages activated with LPS and IFN-γ.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
mouse ... Nos2(18126)
rat ... Nos2(24599)
General description
Anti-Nitric Oxide Synthase, inducible is specific for nitric oxide synthase (NOS), derived from activated mouse macrophage RAW 264.7 cells (iNOS, 130 kDa), but does not recognize NOS derived from brain tissue (bNOS) and endothelial cells (eNOS). The NOS isoform characterized in macrophages is a 130 kDa protein, also known as macrophage NOS (macNOS; mNOS), NOS2, or inducible NOS (iNOS). iNOS is an interferon (IFN)-γ-inducible protein. This gene is located on human chromosome 17q11.
Specificity
Anti-Nitric Oxide Synthase, Inducible is specific for nitric oxide synthase (NOS), derived from activated mouse macrophage RAW 264.7 cells (iNOS, 130 kDa), but does not recognize NOS derived from brain tissue (bNOS) and endothelial cells (eNOS). By immunoblotting of an activated mouse macrophage RAW 264.7 cells extract, the staining of the iNOS band is specifically inhibited by the immunizing peptide.
Immunogen
synthetic peptide corresponding to amino acids 1126-1144 of nitric oxide synthase (NOS) of mouse macrophage origin (iNOS, also termed macNOS) conjugated to KLH. The immunogen sequence is highly conserved in rat iNOS and diverges from the human iNOS.
Application
Anti-Nitric Oxide Synthase, Inducible antibody produced in rabbit has been used in immunoblotting and immunohistochemistry.
Biochem/physiol Actions
Inducible nitric oxide synthase (NOS2) produces nitric oxide (NO), which is expected to be lethal to malaria parasites in vitro.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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