N7660

4-Nitrophenol

chromogenic, liquid

• Synonym(s): p-Nitrophenol solution
• Empirical Formula (Hill Notation): C₆H₅NO₃
• CAS Number: 100-02-7
• Molecular Weight: 139.11
• MDL number: MFCD00007331
• UNSPSC Code: 12352204
• eCl@ss: 39032018
• PubChem Substance ID: 57654462
• NACRES: NA.28

Properties

• product name: 4-Nitrophenol solution, 10 mM
• form: liquid
• concentration: 10 mM
• storage temp.: 2-8°C
• SMILES string: Oc1ccc(cc1)[N+]([O-])=O
• InChI: 1S/C6H5NO3/c8-6-3-1-5(2-4-6)7(9)10/h1-4,8H
• InChI key: BTJIUGUIPKRLHP-UHFFFAOYSA-N
• Gene Information: human ... UGT1A4(54657)

Description

General description

4-Nitrophenol is a phenolic compound present as a chemical intermediate and breakdown product during the production of organophosphorus insecticide derivatives of parathion. It is an intermediate during the synthesis of paracetamol. 4-Nitrophenol is a chromogenic catalytic by product of the synthetic substrate 4-nitrophenyl phosphate. It is majorly used as a reference standard in alkaline phosphatase assay. 4-Nitrophenol is nonbiodegradable and its derivatives are used for herbicide and fungicide production.

Application

4-Nitrophenol solution has been used:

• in the generation of standard curve for quantifying alkaline phosphatase activity of mesenchymal stem cells
• bone marrow stromal cells (BMSCs)
• for quantifying β-hexosaminidase activity in HeLa cells
• as a standard for calorimetric determination of alkaline phosphatase activity at 405 nm
• for assessing the β-hexosamindase activity

Preparation Note

Prepared as a 1.39 mg/ml solution suitable for use as a calibrator in alkaline phosphatase assays (such as Bessey-Lowry-Brock method) using p-nitrophenol phosphate as a substrate.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves