MAK562

Branched Chain Amino Acid Kit

sufficient for 100 colorimetric tests

• Synonym(s): BCAA Assay Kit
• UNSPSC Code: 41116104

Properties

• usage: sufficient for 100 colorimetric tests
• Quality Level: 100
• detection method: colorimetric
• relevant disease(s): endocrinological disorders, diabetes; neurological disorders; gastrointestinal diseases
• storage temp.: −20°C

Description

General description

Leucine, isoleucine and valine, known collectively as branched-chain amino acids (BCAA) are unique amongst the essential amino acids in that they undergo oxidation to a greater extent in the peripheral tissues than in liver1. Concentrations of these amino acids in plasma are altered by moderate and exhausting exercise, by diet and the nutritional status of the individual2.

In this assay, BCAA concentration is determined using a coupled enzyme reaction to catalyze the conversion of a BCAA molecule to its ketone form, which results in the conversion of NAD⁺ to NADH.3 NADH is detected by a colorimetric indicator, with an absorption maximum of 450 nm, proportional to the amount of BCAA present.

Application

BCAA Kit has been used to quantify BCAA in various types of samples, such as food, dietary supplements, blood, serum and cells.

Features and Benefits

Highly sensitive, simple and high-throughput assay for measuring BCAA concentration.
Detection time: only 30 minutes
Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.

Click here to download a calculator excel file

Suitability

Suitable for measuring Branched Chain Amino Acids (BCAA′s) in a variety of biological and organic samples.