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Key Documents

MAK487

Sigma-Aldrich

Lactose Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

Lactose Detection Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

Quality Level

application(s)

agriculture
food and beverages
pharmaceutical

detection method

colorimetric
fluorometric

relevant disease(s)

gastrointestinal diseases

shipped in

wet ice

storage temp.

−20°C

General description

Lactose (C12H22O11) is a disaccharide comprised of galactose and glucose which form a β1-4 glycosidic linkage. Lactose is the major sugar in milk, making up around 2–8 % of milk (by weight).

Features and Benefits

  • Linear detection range of the kit is 17 to 2000 µM lactose for the colorimetric assay and 6 to 100 µM for the fluorometric assay.

Suitability

The kit is suitable for lactose activity determination in milk, food, beverages and other biological samples, as well as for studying the effects of drugs on lactose metabolism.

Principle

The Lactose Assay Kit uses a specific enzyme-coupled reaction in which lactose is cleaved and the resulting galactose forms a colored product. The color intensity at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is directly proportional to the lactose concentration in the sample.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


Certificates of Analysis (COA)

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