MAK154
Fluorometric Sphingomyelin Assay Kit
sufficient for 100 fluorometric tests
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
usage
sufficient for 100 fluorometric tests
detection method
fluorometric
relevant disease(s)
cancer
storage temp.
−20°C
General description
Sphinogmyelin, a sphingolipid consisting of a phospholipid (typically phosphocholine) attached to a ceramide moiety, is a major component of cellular membranes. Sphingomylein is enriched in the exoplasmic leaflet of the cell membrane. Within the cell membrane, sphingomyelin interacts with cholesterol to form lipid rafts. The metabolism of sphingomyelin contributes to cellular signaling via the release of ceramide, a second messenger that participates in multiple cellular signaling pathways. Sphingomyelin accumulates in Niemann-Pick disease due to deficiencies in sphingomyelinase activity.
Suitability
This kit is suitable for the detection of sphingomyelin in a variety of samples such as blood and cell extracts.
Principle
Sphingomyelin is determined by a coupled enzyme assay, in which the hydrolysis of sphingomyelin by sphingomyelinase produces phosphocholine, which results in a fluorescent product (λex =540/λem = 590 nm) proportional to the Sphingomyelin present.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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