M8816

Microsomes from Liver, Pooled

from Monkey(Cynomolgus spp.), male

• UNSPSC Code: 12161501
• NACRES: NB.35

Properties

• biological source: Monkey (Cynomolgus spp.)
• Quality Level: 200
• form: liquid
• specific activity: ≥5000 units/mg protein
• packaging: vial of ~10 mg
• shipped in: dry ice
• storage temp.: −70°C

Description

Application

Microsomes from Liver, Pooled has been used to measure the in vitro stabilities of the prodrugs. It has also been used in microsomal incubation to generate BTX-3 metabolites to serve as references for liquid chromatography with tandem mass spectrometry (LC-MS/MS) analyses of urine.

Biochem/physiol Actions

Liver microsomes are subcellular particles derived from the endoplasmic reticulum of hepatic cells. These microsomes are a rich source of drug metabolizing enzymes, including cytochrome P-450. Microsome pools from various sources are useful in the study of xenobiotic metabolism and drug interactions.

Microsomal cytochrome P450 proteins can catalyze the transfer of a single oxygen atom to endogenous and exogenous compounds, like steroids and phenobarbital, meant for excretion and detoxification.

Unit Definition

One unit will produce 1.0 picomole of 6 beta-hydroxytestosterone per minute at pH 7.4 at 37 deg C.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable