GE17-0599-01
Sephacryl® S-300 HR
Cytiva 17-0599-01, pack of 750 mL
Synonym(s):
Sephacryl S-300 Column
About This Item
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packaging
pack of 750 mL
manufacturer/tradename
Cytiva 17-0599-01
matrix
allyl dextran and N, N’-methylenebisacrylamide
particle size
25-75 μm
avg. part. size
50 μm
cleaning in place
2-13
working range
3-11
suitability
suitable for bioprocess medium
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General description
Sephacryl® High Resolution chromatography media is a highly versatile gel filtration media that offers a wide range of fractionation capabilities. The matrix of Sephacryl® High Resolution media is a cross-linked copolymer of allyl dextran and N,N′-methylene bisacrylamide. This crosslinking gives good rigidity and chemical stability. The narrow particle size distribution, together with steep selectivity curves, results in good preparative characteristics with maintained high resolution. The hydrophilic nature of the media minimizes nonspecific adsorption and maximizes recovery. The excellent resolution and flow characteristics, long-term physical and chemical stability, and ease of handling make Sephacryl® HR the medium of choice for routine purification.
Sephacryl® High Resolution is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As a BioProcess medium, Sephacryl® High Resolution, meets industrial demands with security of supply and comprehensive technical and regulatory support.
Five different Sephacryl® High Resolution chromatography media, with different fractionation ranges, are available:
Sephacryl® S-100 High Resolution is designed for separating peptides and small proteins (fractionation range globular proteins 1 × 103–1 × 105).
Sephacryl® S-200 High Resolution are for purifying antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 5 × 103–2.5 × 105).
Sephacryl® S-300 High Resolution are for purifying antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 1 × 104–1.5 × 106).
Sephacryl® S-400 High Resolution to separate polysaccharides, macromolecules with extended structures and even small particles such as plasmids. (fractionation range globular proteins 2 × 104–8 × 106).
Sephacryl® S-500 High Resolution to separate polysaccharides, macromolecules with extended structures and even small particles such as plasmids (fractionation range Dextrans 4 × 104–2 × 107)
Features and Benefits
- Cost-efficient gel filtration media providing high resolution with fast and easy operation.
- Five different fractionation ranges available in Sephacryl® HR family allows for reproducible separations over a wide molecular weight range.
- Sephacryl® S-300 High Resolution medium is designed for purifying antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 1 × 104–1.5 × 106)
- BioProcess medium supported for industrial applications and well-established in approved processes.
Storage and Stability
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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Protocols
This page provides information about performing an isolation of natural multi-protein complexes for structural and functional studies using classical biochemical methods and products from GE Healthcare.
This page shows how to perform a separation with a sephacryl column from GE Healthcare which are Size Exclusion Chromatography media prepared by covalently cross-linking allyl dextran with N,N’-methylene bisacrylamide.
Column Packing and Preparation for Size Exclusion Chromatography
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