G7509
GABase from Pseudomonas fluorescens
lyophilized powder, Protein ≥30 % by biuret
Synonym(s):
GABase Enzyme, GABase from Pseudomonas, Pseudomonas GABase
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About This Item
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biological source
Pseudomonas fluorescens
Quality Level
Assay
≥30 % (biuret)
form
lyophilized powder
specific activity
≥0.5 units/mg protein
composition
Protein, ≥30% biuret
technique(s)
inhibition assay: suitable
storage temp.
−20°C
General description
Research area: Neuroscience
GABase is a commercially available mixture composed of γ-aminobutyric acid aminotransferase (GABGT) and succinic semialdehyde dehydrogenase (SSDH) obtained from Pseudomonas fluorescens.
GABase is a commercially available mixture composed of γ-aminobutyric acid aminotransferase (GABGT) and succinic semialdehyde dehydrogenase (SSDH) obtained from Pseudomonas fluorescens.
Application
GABase from Pseudomonas fluorescens has been used for GABase assay to quantify the extracellular and intracellular concentration of GABA (γ-aminobutyric acid).
Biochem/physiol Actions
GABase catalyzes the conversion of γ-aminobutyric acid (GABA) to succinic acid in the central nervous system. It is used to measure GABA levels in various biological samples. p-Hydroxybenzaldehyde (HBA) is known to be a potential inhibitor on GABase.
Unit Definition
One unit will convert 1.0 μmole of γ-aminobutyric acid (GABA) to succinic semialdehyde and then to succinate per min with a stoichiometric reduction of 1.0 μmole of NADP+ at pH 8.6 at 25 °C.
Physical form
Partially purified lyophilized powder containing buffer salts and stabilizer
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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