Skip to Content
Merck
All Photos(1)

Key Documents

More Documents

G1881

Sigma-Aldrich

α-Glycerophosphate Dehydrogenase-Triosephosphate Isomerase from rabbit muscle

Type III, ammonium sulfate suspension

Synonym(s):

GDH/TIM, α-GDH-TPI

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Enzyme Commission number:
1.1.1.8 (BRENDA, IUBMB)
MDL number:
MFCD00130488
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

rabbit muscle

Quality Level

200

type

Type III

form

ammonium sulfate suspension

GDH activity

75-200 units/mg protein (biuret)

TPI activity

750-2000 units/mg protein

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

General description

Research area: Neuroscience

α-Glycerophosphate Dehydrogenase-Triosephosphate isomerase (α-GDH-TPI or GDH/TIM) is an enzyme mixture of α-glycerophosphate dehydrogenase (GDH) and triosephosphate isomerase (TPI).

Application

α-Glycerophosphate Dehydrogenase-Triosephosphate Isomerase from rabbit muscle has been used in spectrophotometric aldolase assay and fructose 2,6 bisphosphate assay. It has also been used in erythrocyte transketolase (ETK) activity to study the prevalence of and factors associated with thiamin deficiency in obese.
α-glycerophosphate dehydrogenase was used in 2-deoxy-ribose 5-phosphate aldolase (DERA) cleavage (retroaldol) assay.

Biochem/physiol Actions

α-Glycerophosphate dehydrogenase (α-GPDH) has the ability to produce reactive oxygen species (ROS), that is induced by Ca2+.
α-glycerophosphate dehydrogenase catalyzes the conversion of dihydroxyacetone to glycerol phosphate. α-Glycerophosphate dehydrogenase (α-GPDH) has the ability to produce reactive oxygen species (ROS), that is induced by Ca2+. Triosephosphate isomerase (TPI) is an essential enzyme in the glycolytic pathway, responsible for converting dihydroxyacetone phosphate (DHAP) into glyceraldehyde-3 phosphate (GAP). TPI′s role is crucial in enhancing the efficiency of the catabolic process within glycolysis. However, a deficiency in TPI can lead to glycolytic enzymopathies, rare genetic disorders characterized by neurologic dysfunction and hemolytic anemia.

Packaging

(Sold on the basis of α-GDH units.)

Unit Definition

(α-GDH) One unit will convert 1.0 μmole of dihydroxyacetone phosphate to α-glycerophosphate per min at pH 7.4 at 25 °C.
(TPI) One unit will convert 1.0 μmole of D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.

Physical form

Mixed crystalline suspension in 3.2 M (NH4)2SO4, with 0.05 g/L EDTA, pH 6.0

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

The role of mitochondrial dehydrogenases in the generation of oxidative stress
Adam-Vizi V and Tretter L
Neurochemistry International, 62(5), 757-763 (2013)
C R Hussey et al.
European journal of biochemistry, 80(2), 497-506 (1977-11-01)
A preparation of phosphofructokinase from rabbit skeletal muscle is described which exploits the association-dissociation properties of the enzyme. Phosphofructokinase to prepared is partially phosphorylated and may be fractioned into three distinct species with sedimentation coefficients of 30 S, 18 S
Identification of an anaerobically induced phosphoenolpyruvate-dependent fructose-specific phosphotransferase system and evidence for the Embden-Meyerhof glycolytic pathway in the heterofermentative bacterium Lactobacillus brevis.
Saier M H, et al.
Journal of Bacteriology, 178(1) (1996)
Grace DeSantis et al.
Bioorganic & medicinal chemistry, 11(1), 43-52 (2002-12-07)
2-Deoxyribose-5-phosphate aldolase (DERA, EC 4.1.2.4) catalyzes the reversible aldol reaction between acetaldehyde and D-glyceraldehyde-3-phosphate to generate D-2-deoxyribose-5-phosphate. It is unique among the aldolases as it catalyzes the reversible asymmetric aldol addition reaction of two aldehydes. In order to expand the
Wenguang Liang et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 25(2), 497-504 (2010-10-07)
The pentose phosphate pathway (PPP) confers protection against oxidative stress by supplying NADPH necessary for the regeneration of glutathione, which detoxifies H(2)O(2) into H(2)O and O(2). RPE functions in the PPP, catalyzing the reversible conversion of D-ribulose 5-phosphate to D-xylulose
View All Related Papers

Articles

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service