G1881
α-Glycerophosphate Dehydrogenase-Triosephosphate Isomerase from rabbit muscle
Type III, ammonium sulfate suspension
Synonym(s):
GDH/TIM, α-GDH-TPI
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About This Item
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
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biological source
rabbit muscle
type
Type III
form
ammonium sulfate suspension
GDH activity
75-200 units/mg protein (biuret)
TPI activity
750-2000 units/mg protein
storage temp.
2-8°C
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General description
Research area: Neuroscience
α-Glycerophosphate Dehydrogenase-Triosephosphate isomerase (α-GDH-TPI or GDH/TIM) is an enzyme mixture of α-glycerophosphate dehydrogenase (GDH) and triosephosphate isomerase (TPI).
α-Glycerophosphate Dehydrogenase-Triosephosphate isomerase (α-GDH-TPI or GDH/TIM) is an enzyme mixture of α-glycerophosphate dehydrogenase (GDH) and triosephosphate isomerase (TPI).
Application
α-Glycerophosphate Dehydrogenase-Triosephosphate Isomerase from rabbit muscle has been used in spectrophotometric aldolase assay and fructose 2,6 bisphosphate assay. It has also been used in erythrocyte transketolase (ETK) activity to study the prevalence of and factors associated with thiamin deficiency in obese.
α-glycerophosphate dehydrogenase was used in 2-deoxy-ribose 5-phosphate aldolase (DERA) cleavage (retroaldol) assay.
Biochem/physiol Actions
α-Glycerophosphate dehydrogenase (α-GPDH) has the ability to produce reactive oxygen species (ROS), that is induced by Ca2+.
α-glycerophosphate dehydrogenase catalyzes the conversion of dihydroxyacetone to glycerol phosphate. α-Glycerophosphate dehydrogenase (α-GPDH) has the ability to produce reactive oxygen species (ROS), that is induced by Ca2+. Triosephosphate isomerase (TPI) is an essential enzyme in the glycolytic pathway, responsible for converting dihydroxyacetone phosphate (DHAP) into glyceraldehyde-3 phosphate (GAP). TPI′s role is crucial in enhancing the efficiency of the catabolic process within glycolysis. However, a deficiency in TPI can lead to glycolytic enzymopathies, rare genetic disorders characterized by neurologic dysfunction and hemolytic anemia.
Packaging
(Sold on the basis of α-GDH units.)
Unit Definition
(α-GDH) One unit will convert 1.0 μmole of dihydroxyacetone phosphate to α-glycerophosphate per min at pH 7.4 at 25 °C.
(TPI) One unit will convert 1.0 μmole of D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.
Physical form
Mixed crystalline suspension in 3.2 M (NH4)2SO4, with 0.05 g/L EDTA, pH 6.0
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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