G1019

Gamborg′s Vitamin Solution

1000 ×, liquid, suitable for plant cell culture

• Synonym(s): Gamborg Vitamin Solution, Vitamin Solution
• UNSPSC Code: 10171502
• NACRES: NA.72

Properties

• sterility: sterile-filtered
• form: liquid
• concentration: 1000 ×
• technique(s): cell culture | plant: suitable
• color: colorless
• pH: 5.0-7.0
• application(s): agriculture
• storage temp.: 2-8°C

Description

Application

Gamborg′ B-5 medium described by Gamborg, et al. may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro plant culture of cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis. Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.

Use at a concentration of one ml per liter of prepared medium to achieve the proper final concentration.

Components

Solution contains (mg/ml): 100.0 myo-inositol, 1.0 nicotinic acid, 1.0 pyridoxine hydrochloride, 10.0 thiamine hydrochloride.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable