F2178

Anti-FKHRL1 (FOXO3a) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-AF6q21, Anti-FKHRL1, Anti-FKHRL1P2, Anti-FOXO2, Anti-FOXO3A
• MDL number: MFCD03791550
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: mouse, human
• packaging: antibody small pack of 25 μL
• technique(s): indirect immunofluorescence: 1:200 using paraformaldyde-fixed FKHRL1 transfected 293T cells; microarray: suitable; western blot: 1:2,500 using human FKHRL1 in transfected 293T cell extracts
• UniProt accession no.: O43524
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... FOXO3(2309) ; mouse ... Foxo3(56484)

Description

General description

Forkhead family transcription factor (FKHRL1), also known as FOX3a, is a member of the forkhead transcription factor superfamily. In FKHRL1 (FOX3a), there are three PKB phosphorylation consensus sites, Thr³², Ser²⁵³, and Ser³¹⁵. Thr³² and Ser²⁵³ are phosphorylated by PKB after induction by survival factors such as IGF-1.

Immunogen

thetic peptide corresponding to the N-terminus of human FKHRL1 (amino acids 653-668 with N-terminally added cystein) conjugated to maleimide-activated KLH. This sequence is conserved in human and mouse FKHRL1.

Application

Anti-FKHRL1 (FOXO3a) antibody produced in rabbit has been used in:

• immunohistochemistry
• western blotting
• immunofluorescence staining

Biochem/physiol Actions

Forkhead transcription factor superfamily proteins act as the targets of the phosphoinositide-3-kinase–protein kinase B/Akt (PI3K-PKB) pathway. FKHRL1/FOXO3a modulates the expression of several genes that regulate DNA repair in response to stress at the G2-M checkpoint and aging. Other genes that are regulated by FKHRL1 include mitotic genes such as cyclin B and polo-like kinase (plk). Antibodies reacting specifically with FKHRL1 (FOXO3) may be useful in studying the expression and function of the protein, as well as for correlating their expression pattern with physiological functions or pathological conditions.

Forkhead1 (FOXO-3A or FKHRL-1) functions to protect cells from oxidative stress . Dephosphorylation of FOXO3a stimulates the Fas ligand gene that activates apoptosis in cells . Anti-FKHRL1 is specific for human and mouse FKHRL1. FKHRL1 immunizing peptide specifically inhibits the staining of FKHRL1.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable