E9030

Endoglycoceramidase II from Rhodococcus sp.

aqueous solution

• Synonym(s): EGCase, ceramide glycanase, glycosyl-N-acetyl-sphingosine 1,1-β-D-glucanohydrolase, oligoglycosylglucosyl(1↔1)ceramide glycohydrolase, oligoglycosylglucosylceramide glycohydrolase
• Enzyme Commission number: 3.2.1.123 (BRENDA, IUBMB)
• MDL number: MFCD03456712
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• conjugate: (Lipid-linked)
• form: solution
• mol wt: 58.9 kDa
• shipped in: dry ice
• storage temp.: −20°C

Description

Application

Endoglycoceramidase II from Rhodococcus sp. has been used in a study to assess the differentiation of glycosphingolipid-derived glycan structural isomers by liquid chromatography and mass spectrometry. Endoglycoceramidase II from Rhodococcus sp. has also been used in a study to investigate structural and mechanistic analyses of endo-glycoceramidase II.

Unit Definition

One unit will hydrolyze 1 μmol of asialo-GM1 per min at 37 °C at pH 5.0.

Physical form

Solution in 20 mM sodium acetate buffer, pH 6.0, containing 0.2% BSA and 0.1% Lubrol PX.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves