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Key Documents

PNT2 Cell Line human

Normal prostate epithelium immortalized with SV40., 95012613

Synonym(s):

PNT-2 Cells

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About This Item

UNSPSC Code:
41106514

biological source

human prostate

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human prostate normal, immortalised with SV40

Cell Line Description

Established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from a prostate of a 33 year old male at post mortem. PNT2 cells contain the SV40 genome and express large T protein. They possess a well differentiated morphology with the expression of cytokeratin 8,18 and 19 with the latter being a feature of differentiated luminal cells of the glandular prostate. Cytokeratin 14, a marker of epithelial basal cells, is not expressed. The cells are non-tumourigenic in nude mice.

Application

Biology of prostate cells, model of differentiated prostate epithelial cells.
PNT2 cell line has been used:
  • to assess the effects of Verapamil, an L-type calcium channel blocker on autophagy
  • to assess the cytotoxicity of aqueous dilutions of a universal self-priming dental adhesive (DA)
  • to study the expression of soluble adenylyl cyclase (sAC) in the cells

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 8,13
D16S539: 9
D5S818: 12,13
D7S820: 11
THO1: 6,9.3
TPOX: 8,9
vWA: 17

Culture Medium

RPMI 1640 + 2mM Glutamine + 6 -10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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Certificates of Analysis (COA)

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