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About This Item
UNSPSC Code:
41106514
biological source:
rat liver
growth mode:
Adherent
karyotype:
100 chromosomes
morphology:
Epithelial
products:
Not specified
receptors:
Not specified
relevant disease(s):
cancer
Recommended Products
Product Name
HF1-5, 94101909
biological source
rat liver
growth mode
Adherent
karyotype
100 chromosomes
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat hepatoma Hybrid [H5xFao]
Cell Line Description
HF1-5 is a subclone of HF1 selected for it′s ability to grow in glucose free medium. The entire group of hepatic functions are re-expressed. NB. The cells are difficult to cultivate, they do not like either low or high density and should be transferred frequently even though they grow slowly.
Application
Gene analysis and liver function expression.
Culture Medium
Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Subculture Routine
Split semi-confluent (60%-70%) cultures 1:5 i.e. seeding at 3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 37°C. Saturation density is reached at 5x1000,000 cells/10cm dish.
Other Notes
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