AGS Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89090402, human stomach, Not specified

• UNSPSC Code: 41106514

Properties

• biological source: human stomach
• description: Human Caucasian gastric adenocarcinoma
• packaging: tube of 5 μg 89090402-DNA-5UG; pkg of vial of cells 89090402-1VL
• growth mode: Adherent
• karyotype: Hyperdiploid
• morphology: Not specified
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian gastric adenocarcinoma

Cell Line Description

Derived from an adenocarcinoma of the stomach of a 54 year-old Caucasian female with no prior anti-cancer treatment. AGS cells are persistently infected with parainfluenza virus type 5 (PIV5).

Application

AGS human cell line has been used:

• to study the role of obestatin/G protein-coupled receptor 39 (GPR39) in gastric cancer progression
• to determine expression levels of colon cancer associated transcript -1 (CCAT1)
• to study the adhesion of Lactobacillus sps. to the gastric mucosa

Anti-tumour testing

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 11,13
D5S818: 9,12
D7S820: 10,11
THO1: 6,7
TPOX: 11,12
vWA: 16,17

Culture Medium

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 0.05% EDTA; 5% CO₂; 37°C.

Other Notes

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